Oral microbiome characteristics in children with and without early childhood caries.

Objective: Early childhood caries (ECC) negatively affects children's growth due to its close relation to an imbalance of the oral microbiota. This study aimed to evaluate the distribution of the oral microbiota in children with ECC and healthy individuals.

Methods: The oral microbiota of 20 children with dental caries from both carious teeth (CC cohort) and healthy teeth (CH cohort), and the oral microbiota of 20 healthy control children (HH cohort) were subjected to 16S rDNA sequencing.

Results: The results revealed significant differences between the microbial structure of the CC and CH cohorts of every child with ECC. The most common microbes were , , , and . Specifically, the CC cohort contained , , and , the CH cohort contained , and , and the HH cohort mainly contained , , and . Lastly, we established a random forest model consisting of 10 genera (, , ) which demonstrated promising clinical diagnostic ability (area under the curve (AUC) = 89.8%). These findings indicate that oral microbiota can potentially be used as therapeutic targets or diagnostic markers for the early prediction and prevention of caries in children.