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It is well known that activation of NMDA receptors can trigger long-term synaptic depression (LTD) and that a morphological correlate of this functional plasticity is spine retraction and elimination. Recent studies have led to the surprising conclusion that NMDA-induced spine shrinkage proceeds independently of ion flux and requires the initiation of protein synthesis, highlighting an unappreciated contribution of mRNA translation to non-ionotropic NMDAR signaling. Here we used NMDA-induced spine shrinkage in slices of mouse hippocampus as a readout to investigate this novel modality of synaptic transmission.
View Article and Find Full Text PDF3D-printed educational model for anatomy-driven preparation and retraction cord placement.
J Dent Educ
January 2025
Department of Prosthodontics, Martin Luther University Halle-Wittenberg, Halle, Germany.
The impact of retraction cords on the gingival margin level: A randomized clinical trial.
J Prosthodont
January 2025
Department of Oral and Maxillofacial Surgery, Oral Medicine and Periodontology, School of Dentistry, University of Jordan, Amman, Jordan.
Purpose: To examine the impact of placing plain and impregnated retraction cords for two different retraction times on the postoperative gingival margin level and periodontal health.
Materials And Methods: A total of 40 endodontically treated mandibular first molars were selected and randomly allocated into four groups (n = 10/group); A: plain retraction cord for 10 min, B: plain retraction cord, 20 min, C: impregnated (25% aluminum chloride (AlCl) retraction cord, 10 min, D: impregnated retraction cord (25% AlCl), 20 min. Intraoral digital scans were acquired, and periodontal parameters (Plaque index [PI], Bleeding on probing [BOP], and probing depth [PD]) were assessed at baseline, 7 days, and 28 days after retraction.
Development of a simplified platelet cryopreservation method: An in vitro investigation of reducing the DMSO concentration to allow administration without its pre-transfusion removal.
Vox Sang
January 2025
Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia.
Background And Objectives: The most widely used method of platelet cryopreservation requires the addition of 5%-6% dimethylsulphoxide (DMSO), followed by its pre-freeze removal via centrifugation, to minimize toxicity. However, this adds complexity to the pre-freeze and post-thaw processing. Accordingly, the aim of this study was to simplify platelet cryopreservation by reducing the DMSO concentration and omitting the requirement for pre-transfusion removal.
View Article and Find Full Text PDF[Beyond premature apnea pauses: congenital myotonic dystrophy type 1].
Andes Pediatr
October 2024
Departamento de Neuropediatría, Hospital Fundación Alcorcón, Madrid, España.
Unlabelled: Congenital myotonic dystrophy type 1 (DM1) is a rare entity that can pose a diagnostic challenge, especially if other processes such as prematurity coexist.
Objective: to describe the typical presentation of congenital DM1 and thus increase diagnostic suspicion.
Clinical Case: A 29-week preterm female newborn who required non-invasive mechanical ventilation until 41 weeks postmenstrual age; she presented with apnea requiring manual ventilation with a self-inflating bag and cardiac massage.
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