AI Article Synopsis
- Cryptosporidium parvum is a significant parasite that causes severe diarrhea in humans and animals, and the role of the calcium-binding protein calmodulin (CaM) in its development is not well understood.
- Researchers expressed the CaM encoded by the cgd2_810 gene in E. coli and found that its transcription peaked 36 hours post-infection, with the protein predominantly around the nucleus in various stages of the parasite.
- Using an anti-CpCaM antibody, the study demonstrated a 30.69% reduction in sporozoite invasion, suggesting that CpCaM plays an important role in the growth and infection process of C. parvum.
Article Abstract
Cryptosporidium parvum is an important apicomplexan parasite causing severe diarrhea in both humans and animals. Calmodulin (CaM), a multifunctional and universal calcium-binding protein, contributes to the growth and development of apicomplexan parasites, but the role of CaM in C. parvum remains unknown. In this study, the CaM of C. parvum encoded by the cgd2_810 gene was expressed in Escherichia coli, and the biological functions of CpCaM were preliminarily investigated. The transcriptional level of the cgd2_810 gene peaked at 36 h post infection (pi), and the CpCaM protein was mainly located around the nucleus of the whole oocysts, in the middle of sporozoites and around the nucleus of merozoites. Anti-CpCaM antibody reduced the invasion of C. parvum sporozoites by 30.69%. The present study indicates that CpCaM is potentially involved in the growth of C. parvum. Results of the study expand our knowledge on the interaction between host and Cryptosporidium.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00436-023-07803-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!