Integrative ATAC-seq and RNA-seq analyses of IPEC-J2 cells reveals porcine transcription and chromatin accessibility changes associated with F18ac inhibited by .

is the main cause of postweaning diarrhea in pigs, leading to economic loss. As a probiotic, has been used to inhibit in clinical applications; however, its integrative interactions with hosts remain unclear, especially in pigs. Here, we found that effectively inhibited F18ac adhering to porcine IPEC-J2 cells, and explored the genome-wide transcription and chromatin accessibility landscapes of IPEC-J2 cells by RNA-seq and ATAC-seq. The results showed that some key signal transduction pathways, such as PI3K-AKT and MAPK signaling pathways, were enriched in the differentially expressed genes (DEGs) between F18ac treatment with and without groups. However, we found less overlap between RNA-seq and ATAC-seq datasets; we speculated that this might be caused by histones modification through ChIP-qPCR detection. Furthermore, we identified the regulation of the actin cytoskeleton pathway and a number of candidate genes (, , and ) that might be associated with the inhibition of F18ac adherence to IPEC-J2 cells by . In conclusion, we provide a valuable dataset that can be used to seek potential porcine molecular markers of F18ac pathogenesis and antibacterial activity, and to guide the antibacterial application of .