Quantitative analysis of zebrafish cerebral vasculature is essential for the study of vascular development and disease. We developed a method to accurately extract the cerebral vasculature topological parameters of transgenic zebrafish embryos. The intermittent and hollow vascular structures of transgenic zebrafish embryos, obtained from 3D light-sheet imaging, were transformed into continuous solid structures with a filling-enhancement deep learning network. The enhancement enables the extraction of 8 vascular topological parameters accurately. Quantitation of the zebrafish cerebral vasculature vessels with the topological parameters show a developmental pattern transition from 2.5 to 5.5 dpf.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979664 | PMC |
| http://dx.doi.org/10.1364/BOE.484351 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cerebral perfusion correlates with amyloid deposition in patients with mild cognitive impairment due to Alzheimer's disease.
J Prev Alzheimers Dis
February 2025
Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China, 154 Anshan Road Tianjin 300052, PR China; Department of Neurology, Tianjin Medical University General Hospital Airport Site, Tianjin 300052, PR China. Electronic address:
Background: Changes in cerebral blood flow (CBF) may contribute to the initial stages of the pathophysiological process in patients with Alzheimer's disease (AD). Hypoperfusion has been observed in several brain regions in patients with mild cognitive impairment (MCI). However, the clinical significance of CBF changes in the early stages of AD is currently unclear.
View Article and Find Full Text PDFModeling of Blood Flow Dynamics in Rat Somatosensory Cortex.
Biomedicines
December 2024
Blue Brain Project, École Polytechnique Fédérale de Lausanne (EPFL), Campus Biotech, 1202 Geneva, Switzerland.
The cerebral microvasculature forms a dense network of interconnected blood vessels where flow is modulated partly by astrocytes. Increased neuronal activity stimulates astrocytes to release vasoactive substances at the endfeet, altering the diameters of connected vessels. Our study simulated the coupling between blood flow variations and vessel diameter changes driven by astrocytic activity in the rat somatosensory cortex.
View Article and Find Full Text PDFNotch dimerization provides robustness against environmental insults and is required for vascular integrity.
PLoS One
January 2025
Division of Developmental Biology, Department of Pediatrics, University of Cincinnati College of Medicine and Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
The Notch intracellular domain (NICD) regulates gene expression during development and homeostasis in a transcription factor complex that binds DNA either as monomer, or cooperatively as dimers. Mice expressing Notch dimerization-deficient (NDD) alleles of Notch1 and Notch2 have defects in multiple tissues that are sensitized to environmental insults. Here, we report that cardiac phenotypes and DSS (Dextran Sodium Sulfate) sensitivity in NDD mice can be ameliorated by housing mice under hypo-allergenic conditions (food/bedding).
View Article and Find Full Text PDFA novel method for detecting intracranial pressure changes by monitoring cerebral perfusion via electrical impedance tomography.
Fluids Barriers CNS
January 2025
Department of Biomedical Engineering, Air Force Medical University, Xi'an, China.
Background: Acute and critical neurological diseases are often accompanied with elevated intracranial pressure (ICP), leading to insufficient cerebral perfusion, which may cause severe secondary lesion. Existing ICP monitoring techniques often fail to effectively meet the demand for real-time noninvasive ICP monitoring and warning. This study aimed to explore the use of electrical impedance tomography (EIT) to provide real-time early warning of elevated ICP by observing cerebral perfusion.
View Article and Find Full Text PDFRetinal vasculature changes as indicators of imaging signs of cerebral small vessel disease: A community-based, cross-sectional study of older adults.
J Clin Neurosci
January 2025
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; Hubei Key Laboratory of Neural Injury and Functional Reconstruction, Huazhong University of Science and Technology, China. Electronic address:
Objectives: This study investigated the correlation between retinal vasculature and cerebral small vessel disease (CSVD) imaging markers, providing new evidence for the retina-brain association.
Methods: Two hundred and thirty-nine participants aged 55-85 were enrolled in the study. CSVD indicators, encompassing white matter hyperintensities (WMHs), lacunes (LAs), cerebral microbleeds (CMBs), and enlarged perivascular spaces (EPVSs), were assessed.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!