The first report describes features of the chloroplast genome of .

Genomic studies not only help researcher not only to identify genomic features in organisms, but also facilitate understanding of evolutionary relationships. Species in the genus have medicinal benefits, and one of them is which is used to treat various diseases. This report investigates the nucleotides and genic features of chloroplast genome of and trying to clarify the evolutionary relationship with and family Solanaceae. We found that the total size of genome was 153.771 kb (the smallest chloroplast genome in genus Withania). A large single-copy region (91.285 kb), a small single-copy region (18.373 kb) form the genomic region, and are distinct from each other by a large inverted repeat (22.056 kb). 137 chloroplast genes are found including 4 rRNAs, 38 tRNAs and 83 protein-coding genes. The chloroplast genome as well as four closest relatives was compared for features such as structure, nucleotide composition, simple sequence repeats (SSRs) and codon bias. Compared to other species, has unique characteristics. It has the smallest chloroplast genome of any species, isoleucine is the major amino acid, and tryptophan is the minor, In addition, there are no and genes, fourth, there are only fifteen replicative genes, while in most other species there are more. Using fast minimum evolution and neighbor joining, we have reconstructed the trees to confirm the relationship with other Solanacaea species. The chloroplast genome is submitted under accession no. ON153173.