Effect of alkynyloxy derivatives of lawsone as an antifungal spray for acrylic denture base: An study.

Objective: The purpose of this study was to (i) synthesize and develop an alkynyloxy derivative of lawsone as an antifungal spray and (ii) assess the antifungal spray's effectiveness in reducing the viability of () on polymethylmethacrylate (PMMA) specimens.

Methods: Lawsone methyl ether (LME) and its derivative, 2-(prop-2-ynyloxy)naphthalene-1,4-dione (compound ) were synthesized and characterized. The synthetic compounds were screened for antimicrobial activities against using the microtiter broth dilution method to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Compound was further formulated as an antifungal spray in three concentrations (100, 200, and 400 μg/mL). biofilms were developed for 48 h on PMMA specimens. The efficacy of using an antifungal spray for 1 and 3 min to remove biofilm was assessed using colony counting and scanning electron microscopy (SEM). Chlorhexidine gluconate (CHX), polident®, and distilled water were used as positive and negative control cleansing solutions, respectively.

Results: LME and compound showed comparable inhibition against with a MIC of 25 μg/mL and MFC of 50 μg/mL. For immediate treatment, was not detected on PMMA specimens when expose to 2% CHX and compound (100, 200, and 400 μg/mL) antifungal spray for 3 min. However, after recolonization, a small number of viable cells were observed in denture soaked in compound antifungal spray for 3 min group. Following recolonization, polident® and distilled water had comparable viable cell counts of to the no treatment group. Scanning electron microscope (SEM) images revealed that CHX, polident®, and compound caused cell damage in various forms.

Conclusion: Denture spray containing synthetic alkynyloxy derivative of lawsone is a promising antifungal agent for biofilm removal from the PMMA surface.