AI Article Synopsis
- Histone H2A monoubiquitination (H2Aub1) helps regulate gene expression and maintain cellular identity in eukaryotes, particularly in Arabidopsis.
- The study identifies that the cohesin subunits AtSYN4 and AtSCC3 assist in recruiting AtBMI1, a key component of the polycomb repressive complex 1, to specific genomic areas for H2Aub1 modification.
- Findings show that AtSYN4 directly interacts with the G-box motif, guiding H2Aub1 to active transcription sites, thus unveiling a new mechanism of gene regulation.
Article Abstract
Histone H2A monoubiquitination (H2Aub1) functions as a conserved posttranslational modification in eukaryotes to maintain gene expression and guarantee cellular identity. Arabidopsis H2Aub1 is catalyzed by the core components AtRING1s and AtBMI1s of polycomb repressive complex 1 (PRC1). Because PRC1 components lack known DNA binding domains, it is unclear how H2Aub1 is established at specific genomic locations. Here, we show that the Arabidopsis cohesin subunits AtSYN4 and AtSCC3 interact with each other, and AtSCC3 binds to AtBMI1s. H2Aub1 levels are reduced in atsyn4 mutant or AtSCC3 artificial microRNA knockdown plants. ChIP-seq assays indicate that most binding events of AtSYN4 and AtSCC3 are associated with H2Aub1 along the genome where transcription is activated independently of H3K27me3. Finally, we show that AtSYN4 binds directly to the G-box motif and directs H2Aub1 to these sites. Our study thus reveals a mechanism for cohesin-mediated recruitment of AtBMI1s to specific genomic loci to mediate H2Aub1.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984397 | PMC |
| http://dx.doi.org/10.1038/s41467-023-36788-3 | DOI Listing |
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