Objective: T cell immunoglobulin and mucin-containing protein-3 (TIM-3) is an important immune checkpoint, but its role in lung cancer is still not clear. In this study, we investigated TIM-3 protein expression and its correlation with TNF- and IFN- by examining the tissues of patients with lung adenocarcinoma.
Methods: We detected the mRNA quantity of TIM-3, TNF-, and IFN- in 40 surgically resected specimens from patients with lung adenocarcinoma by real-time quantitative polymerase chain reaction (qRT-PCR). The protein expression of TIM-3, TNF-, and IFN- was assessed in normal tissues, paracarcinoma tissues, and tumor tissues by western blotting, respectively. The relevance between the expression and clinicopathological information of the patients was analyzed.
Results: The results showed that the expression level of TIM-3 was higher in tumor tissues than normal tissues and paracancerous tissues ( < 0.05). On the contrary, the expression of TNF- and IFN- in tumor tissues was lower than normal tissues and paracarcinoma tissues ( < 0.05). However, the expression levels of IFN- mRNA were not observed to be significantly different between cancerous tissues and adjacent tissues. While TIM-3 protein expression in cancer tissues of patients with lymph node metastasis was higher than in patients without metastasis, the expression of TNF- and IFN- was lower ( < 0.05). Importantly, the expression of TIM-3 was negatively correlated with the expression of TNF- and IFN-, and the expression of TNF- was found to be positively correlated with IFN- in the patient.
Conclusion: The high expression of TIM-3, the low expression of TNF- and IFN-, and the synergistic effect of TNF- and IFN- in patients with lung adenocarcinoma were closely related to poor clinicopathological characteristics. Overexpression of TIM-3 may play an important role in the relationship between TNF- and IFN- secretion and poor clinicopathological characteristics.
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| http://dx.doi.org/10.1155/2023/2352945 | DOI Listing |
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