Resveratrol Prevents -Induced Sepsis by Attenuating Necroptosis.

Objective: This study investigated how the natural phytophenol and potent SIRT1 activator resveratrol (RSV) regulate necroptosis during induced sepsis and the potential mechanism.

Methods: The effect of RSV on cytolysin (VVC)-induced necroptosis was analyzed using CCK-8 and Western blot assays. Enzyme-linked immunosorbent assays and quantitative real-time polymerase chain reaction, western blot, and immunohistochemistry and survival analyses were performed to elucidate the effect and mechanism of RSV on necroptosis in a -induced sepsis mouse model.

Results: RSV relieved necroptosis induced by VVC in RAW264.7 and MLE12 cells. RSV also inhibited the inflammatory response, had a protective effect on histopathological changes, and reduced the expression level of the necroptosis indicator pMLKL in peritoneal macrophages, lung, spleen, and liver tissues of -induced septic mice . Pretreatment with RSV downregulated the mRNA of the necroptosis indicator and protein expression in peritoneal macrophages and tissues of -induced septic mice. RSV also improved the survival of -induced septic mice.

Conclusion: Our findings collectively demonstrate that RSV prevented -induced sepsis by attenuating necroptosis, highlighting its potency in the clinical management of -induced sepsis.