Methyl methacrylate (MMA) is an important petrochemical with many applications. However, its manufacture has a large environmental footprint. Combined biological and chemical synthesis (semisynthesis) may be a promising alternative to reduce both cost and environmental impact, but strains that can produce the MMA precursor (citramalate) at low pH are required. A non-conventional yeast, , may prove ideal, as it can survive extremely low pH. Here, we demonstrate the engineering of for citramalate production. Using sequence similarity network analysis and subsequent DNA synthesis, we selected a more active citramalate synthase gene () variant for expression in . We then adapted a piggyBac transposon system for that allowed us to simultaneously explore the effects of different gene copy numbers and integration locations. A batch fermentation showed the genome-integrated- strains produced 2.0 g/L citramalate in 48 h and a yield of up to 7% mol citramalate/mol consumed glucose. These results demonstrate the potential of as a chassis for citramalate production.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9969067 | PMC |
http://dx.doi.org/10.1016/j.mec.2023.e00220 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!