Turn-On Fluorescent Probe Based on a Dansyl Triarginine Peptide for Ganglioside Imaging.

ACS Org Inorg Au

Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.

Published: December 2021

Gangliosides play pivotal biological roles in the animal cell membranes, and it is vital to develop fluorescent probes for imaging them. To date, various artificial receptors for ganglioside imaging have been developed; however, turn-on fluorescence imaging for gangliosides with high contrast has not been achieved. We developed a simple fluorescent probe on the basis of a dansyl triarginine peptide for turn-on ganglioside imaging on the liposome membrane. The probe bound to monosialyl gangliosides and other anionic lipids with association constants was 10 M, which enhanced from 6-fold to 7-fold the fluorescence intensity. Upon binding to monosialyl ganglioside-containing giant liposomes, the turn-on probe selectively enhanced the fluorescence intensity compared with the other anionic lipids. This simple peptide probe for turn-on fluorescence imaging of gangliosides would provide a novel molecular tool for chemical biology.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954261PMC
http://dx.doi.org/10.1021/acsorginorgau.1c00013DOI Listing

Publication Analysis

Top Keywords

ganglioside imaging
12
imaging gangliosides
12
fluorescent probe
8
dansyl triarginine
8
triarginine peptide
8
turn-on fluorescence
8
fluorescence imaging
8
anionic lipids
8
fluorescence intensity
8
imaging
6

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!