To quantitatively evaluate the inhibition of human DNA repair proteins APE1 and MTH1 by dextran-coated γ-FeO ultrasmall superparamagnetic iron oxide nanoparticles (dUSPIONs). : Liquid chromatography-tandem mass spectrometry with isotope-dilution was used to measure the expression levels of APE1 and MTH1 in MCL-5 cells exposed to increasing doses of dUSPIONs. The expression levels of APE1 and MTH1 were measured in cytoplasmic and nuclear fractions of cell extracts. APE1 and MTH1 expression was significantly inhibited in both cell fractions at the highest dUSPION dose. The expression of MTH1 was linearly inhibited across the full dUSPION dose range in both fractions. These findings warrant further studies to characterize the capacity of dUSPIONs to inhibit other DNA repair proteins and .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031551 | PMC |
| http://dx.doi.org/10.2217/nnm-2022-0204 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metal coordination micelles for anti-cancer treatment by gene-editing and phototherapy.
J Control Release
May 2023
Key Laboratory of Systems Bioengineering (Ministry of Education), Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China. Electronic address:
CRISPR-Cas9 is a central focus of the emerging field of gene editing and photodynamic therapy (PDT) is a clinical-stage ablation modality combining photosensitizers with light irradiation. But metal coordination biomaterials for the applications of both have rarely been investigated. Herein, Chlorin-e6 (Ce6) Manganese (Mn) coordination micelles loaded with Cas9, termed Ce6-Mn-Cas9, were developed for augmented combination anti-cancer treatment.
View Article and Find Full Text PDFInhibition of human APE1 and MTH1 DNA repair proteins by dextran-coated γ-FeO ultrasmall superparamagnetic iron oxide nanoparticles.
Nanomedicine (Lond)
November 2022
Biosystems & Biomaterials Division, National Institute of Standards & Technology, Gaithersburg, MD 20899, USA.
To quantitatively evaluate the inhibition of human DNA repair proteins APE1 and MTH1 by dextran-coated γ-FeO ultrasmall superparamagnetic iron oxide nanoparticles (dUSPIONs). : Liquid chromatography-tandem mass spectrometry with isotope-dilution was used to measure the expression levels of APE1 and MTH1 in MCL-5 cells exposed to increasing doses of dUSPIONs. The expression levels of APE1 and MTH1 were measured in cytoplasmic and nuclear fractions of cell extracts.
View Article and Find Full Text PDFOxidative stress and 8-oxoguanine repair are enhanced in colon adenoma and carcinoma patients.
Mutagenesis
September 2010
Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
Oxidative stress is involved in the pathogenesis of colon cancer. We wanted to elucidate at which stage of the disease this phenomenon occurs. In the examined groups of patients with colorectal cancer (CRC, n = 89), benign adenoma (AD, n = 77) and healthy volunteers (controls, n = 99), we measured: vitamins A, C and E in blood plasma, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanine (8-oxoGua) in leukocytes and urine, leukocyte 8-oxoGua excision activity, mRNA levels of APE1, OGG1, 8-oxo-7,8-dihydrodeoxyguanosine 5'-triphosphate pyrophosphohydrolase (MTH1) and OGG1 polymorphism.
View Article and Find Full Text PDFHeterogeneous molecular mechanisms underlie attenuated familial adenomatous polyposis.
Genet Med
December 2007
Department of Genetics and Microbiology, University of Pavia, Pavia, Italy.
Article Synopsis
- * Researchers analyzed 26 patients with multiple colorectal adenomas who tested negative for APC mutations, leading to the discovery of an APC gene deletion in one patient and various MUTYH mutations in others.
- * The findings suggest that patients with attenuated polyposis may have a diverse genetic background, and it emphasizes the need for thorough genetic testing and clinical management for affected families.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!