The baculoviral chitinase (CHIA) and cathepsin (V-CATH) enzymes promote terminal insect host liquefaction, which aids viral progeny dissemination. Recombinant Autographa californica nucleopolyhedrovirus (AcMNPV)-derived viruses were previously generated with reprogrammed transcription by replacing the native promoter with the AcMNPV () or core protein () promoter sequences, but of both these -reprogrammed viruses lacked transcription and V-CATH enzymatic activity. Here, we report that dual / promoter reprogramming of the adjacent genes resulted in modulated temporal transcription of both genes without impacting infectious budded virus production. These promoter changes increased CHIA and V-CATH enzyme activities in infected -derived cultured cells and larvae. In addition, larvae infected with the dual reprogrammed virus had earlier mortalities and liquefaction. This recombinant baculovirus, lacking exogenous genomic elements and increased expression levels, may be desirable for and amenable to producing enhanced baculovirus-based biopesticides.
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| http://dx.doi.org/10.3390/v15020503 | DOI Listing |
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