[Effect of VIT1/VIT2 overexpression on Fe and Cd accumulation in rice endosperm].

Iron (Fe) deficiency and excess cadmium (Cd) in rice grain are important problems to be solved in agricultural production. Previous studies have shown that OsVIT1 and OsVIT2 are vacuolar iron transporters. In this study, wild-type ZH11 was selected as the background material and and were overexpressed in endosperm by using endosperm specific promoter . Field experiments were conducted to study the effect of and overexpression on Fe and Cd accumulation in different parts of rice. The results showed that overexpression in endosperm significantly reduced Fe content in grain by about 50%, while significantly increased zinc (Zn) and copper (Cu) contents in straw and Cu content in grain. overexpression in endosperm significantly decreased Fe and Cd contents in grain by about 50%, and significantly increased Fe content in straw by 45%-120%. Overexpression of and in endosperm did not affect the agronomic traits of rice. In conclusion, and overexpression in endosperm reduced Fe accumulation in rice grain, which did not achieve the expected effect. overexpression in endosperm also decreased Cd accumulation in grain and increased Fe accumulation in straw, which provided reference for iron biofortification and cadmium reduction in rice.