In this study, a ligand fishing method was developed to screen potential indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors from coffee extracts by immobilization of IDO1 enzyme on amino-modified magnetic nanoparticles combined with UHPLC-Q-TOF-MS/MS analysis. Parameters including enzyme concentration, immobilization time, the pH of glutaraldehyde and the amount of magnetic nanoparticles were optimized. The results indicated that immobilized IDO1 could be reused 5 times and was stable during storage for 7 days. Several IDO1 ligands were captured by incubating immobilized IDO1 with coffee extract, of which 10 showed an obvious difference comparing to non-conjugated bare nanoparticles. inhibitory activity was further performed by CE analysis, in which ferulic acid and chlorogenic acid had better IDO1 inhibitory activity, with IC value of 113.7 μM and 307.5 μM. These results demonstrate that this method provides an effective platform for identifying and screening IDO1 inhibitors from natural products.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945408 | PMC |
| http://dx.doi.org/10.1016/j.fochx.2023.100591 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Green Sol-Gel Synthesis of Iron Oxide Nanoparticles for Magnetic Hyperthermia Applications.
Pharmaceutics
December 2024
i3N and Department of Physics, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Background/objectives: The unique properties of iron oxide nanoparticles have attracted significant interest within the biomedical community, particularly for magnetic hyperthermia applications. Various synthesis methods have been developed to optimize these nanoparticles.
Methods: In this study, we employed a powdered coconut water (PCW)-assisted sol-gel method to produce magnetite nanoparticles for the first time.
Global Identification of Anti-Melanoma Cellular Targets by Photochemically Induced Coupling of Reactions on the Surface of Magnetic Particles.
Pharmaceutics
December 2024
College of Pharmacy, Xinjiang Second Medical College, Karamay 834000, China.
, an active component of Arnebia euchroma (Royle) Johnst., has remarkable pharmacological effects, particularly in its anti-tumour activity. Nonetheless, the specific targets and mechanisms of action remain to be further explored.
View Article and Find Full Text PDFSwitchable Solvent for Separation and Extraction of Lignin from Lignocellulose Biomass: An Investigation of Chemical Structure and Molecular Weight.
Polymers (Basel)
December 2024
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Lignin, the most abundant natural aromatic polymer, holds considerable promise for applications in various industries. The primary obstacle to the valorization of lignin into useful materials is its low molecular weight and diminished chemical reactivity, attributable to its intricate structure. This study aimed to treat lignocellulosic biomass using a switchable solvent (DBU-HexOH/HO) derived from the non-nucleophilic superbase 1,8-diazabicyclo [5.
View Article and Find Full Text PDFMagnetic Composite Carbon from Microcrystalline Cellulose to Tackle Paracetamol Contamination: Kinetics, Mass Transfer, Equilibrium, and Thermodynamic Studies.
Polymers (Basel)
December 2024
Institute of Chemistry, Federal University of Rio Grande do Sul (UFRGS), Bento Gonçalves 9500, Porto Alegre 90010-150, RS, Brazil.
This study reported a one-spot preparation of magnetic composite carbon (MCC@Fe) from microcrystalline cellulose (MC). The pure cellulose was impregnated in iron (III) chloride solution and carbonized at 650 °C. The MCC@Fe composite adsorbent underwent various characterization techniques.
View Article and Find Full Text PDFPreparation of Magnetic Hemicellulosic Composite Microspheres and Adsorption of Copper Ions.
Polymers (Basel)
December 2024
Research Centre of Advanced Chemical Engineering, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an 710129, China.
In this study, the fabrication of magnetic hemicellulosic composite microspheres and the adsorption of copper ions are explored. The microspheres were prepared by the micro-emulsion technique, using FeO nanoparticles and hemicellulose extracted from wheat straw with the ionic liquid B[mim]Cl as a solvent. FeO nanoparticles, synthesized through coprecipitation, were evenly encapsulated within the hemicellulosic microspheres.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!