Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing.

Moscow Univ Biol Sci Bull

Microbiology Department, Biological Faculty, Moscow State University, 119234 Moscow, Russia.

Published: February 2023

The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and spp. in different ratios) and different types of yeast of the genus (family ). Acetic acid bacteria and a small percentage of yeast were detected in the KG from Tibet, and yeast was detected in the KG from Ossetia.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940072PMC
http://dx.doi.org/10.3103/S0096392522040010DOI Listing

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