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A novel subgenotype I3 of hepatitis B virus in Guangxi, China: a 15-year follow-up study. | LitMetric

AI Article Synopsis

  • Research identified a new genotype (Genotype I) of hepatitis B virus using genome sequencing from Vietnam and Laos, but its long-term evolution has not been studied until now.
  • A 15-year analysis using NGS and clone-based sequencing of a healthy HBsAg carrier showed several strains, with most belonging to subgenotype I1, but some forming a new cluster, indicating potential recombination with subgenotype C.
  • Findings suggest the emergence of a new subgenotype, provisionally named HBV subgenotype I3, based on the genetic patterns observed and the disappearance of certain strains, showcasing ongoing molecular evolution of HBV.

Article Abstract

Genotype I of hepatitis B virus (HBV) was proposed recently following sequencing of complete HBV genomes from Vietnam and Laos. However, its long-term molecular evolution is unknown. The objectives of this study were to study the molecular evolution of this genotype from an asymptomatic HBsAg carrier from the Long An cohort over a 15-year period was studied using both NGS and clone-based sequencing. The number of complete genome sequences obtained in 2004, 2007, 2013, and 2019 are 17, 20, 19, and 10, respectively. All strains belong to subgenotype I1, except for six (five from 2007 and one from 2019) and 8 further strains from 2007 which form a cluster branching out from other subgenotype I sequences, supported by a 100% bootstrap value. Based on complete genome sequences, all of the estimated intragroup nucleotide divergence values between these strains and HBV subgenotypes I1-I2 exceed 4%. These strains are recombinants between genotype I1 and subgenotype C but the breakpoints vary. The median intrahost viral evolutionary rate in this carrier was 3.88E-4 substitutions per site per year. The Shannon entropy (Sn) ranged from 0.55 to 0.88 and the genetic diversity, D, ranged from 0.0022 to 0.0041. In conclusion, our data provide evidence of novel subgenotypes. Considering that the 8 strains disappeared after 2007, while one of the 6 strains appears again in 2019, we propose these 6 strains as a new subgenotype, provisionally designated HBV subgenotype I3 and the 8 strains as aberrant genotype.

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Source
http://dx.doi.org/10.1007/s11262-023-01980-6DOI Listing

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