Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by HO.

Talanta

Ministry of Education Key Laboratory for Analysis Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR China. Electronic address:

Published: May 2023

Hyaluronidase (HAase) is a potential tumor biomarker for diseases of the digestive tract and nervous system, the development of simple and sensitive techniques for HAase determination is urgent needed. Gold nanorods (Au NRs) can be etched by HO with high efficiency and display color changing. In this work, a HAase-responsive hydrogel system had been designed and the amount of HO spilled from the system had a close relationship with the amount of HAase, then the spilled HO had been applied to etch Au NRs. The color change of the solution was used to realize semi-quantitative determination of HAase. Furthermore, the longitudinal peak shift of Au NRs had a linear correlation with the concentration of HAase in the range of 10-60 U/mL (within 40 min) and the limit of detection (LOD) was 3.8 U/mL (S/N = 3), which can be used to realize accurate quantitative analysis of HAase. The proposed method has been applied to monitor HAase in serum of pancreatic cancer patients with satisfied results.

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Source
http://dx.doi.org/10.1016/j.talanta.2023.124367DOI Listing

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