Antiparasitic Meroterpenoids Isolated from CF-080171.

is a fungal genus from which a wide range of diverse biologically active compounds have been isolated. A CF-080171 extract was identified to exhibit potent activity against 3D7 and Tulahuen whole parasites in a high-throughput screening (HTS) campaign of microbial extracts from the Fundación MEDINA's collection. Bioassay-guided isolation of the active metabolites from this extract afforded eight new meroterpenoids of varying potencies, namely, memnobotrins C-E (-), a glycosylated isobenzofuranone (), a tricyclic isobenzofuranone (), a tetracyclic benzopyrane (), a tetracyclic isobenzofuranone (), and a pentacyclic isobenzofuranone (). The structures of the isolated compounds were established by (+)-ESI-TOF high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Compounds , and exhibited potent antiparasitic activity against 3D7 (EC 0.04-0.243 μM) and Tulahuen (EC 0.266-1.37 μM) parasites, as well as cytotoxic activity against HepG2 tumoral liver cells (EC 1.20-4.84 μM). The remaining compounds (, -) showed moderate or no activity against the above-mentioned parasites and cells.