AI Article Synopsis
- Sonoporation using ultrasound and microbubbles enhances the delivery of therapeutic molecules across biological barriers, specifically targeting the inner ear via the round window membrane.
- A study assessing the safety of this method in six ewes found no hearing loss or harmful heating effects after the procedure.
- Metabolomic analysis showed no significant differences in perilymph samples between treated and control ears, indicating that sonoporation is safe for the inner ear.
Article Abstract
Sonoporation using microbubble-assisted ultrasound increases the permeability of a biological barrier to therapeutic molecules. Application of this method to the round window membrane could improve the delivery of therapeutics to the inner ear. The aim of this study was to assess the safety of sonoporation of the round window membrane in a sheep model. To achieve this objective, we assessed auditory function and cochlear heating, and analysed the metabolomics profiles of perilymph collected after sonoporation, comparing them with those of the control ear in the same animal. Six normal-hearing ewes were studied, with one sonoporation ear and one control ear for each. A mastoidectomy was performed on both ears. On the sonoporation side, Vevo MicroMarker microbubbles (MBs; VisualSonics-Fujifilm, Amsterdam, The Netherlands) at a concentration of 2 × 10 MB/mL were locally injected into the middle ear and exposed to 1.1 MHz sinusoidal ultrasonic waves at 0.3 MPa negative peak pressure with 40% duty cycle and 100 μs interpulse period for 1 min; this was repeated three times with 1 min between applications. The sonoporation protocol did not induce any hearing impairment or toxic overheating compared with the control condition. The metabolomic analysis did not reveal any significant metabolic difference between perilymph samples from the sonoporation and control ears. The results suggest that sonoporation of the round window membrane does not cause damage to the inner ear in a sheep model.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9964975 | PMC |
| http://dx.doi.org/10.3390/pharmaceutics15020442 | DOI Listing |
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