AI Article Synopsis
- Frequent emergence of SARS-CoV-2 variants, especially the Delta variant, complicates COVID-19 prevention and treatment efforts, leading to a decline in focused research on Delta.
- Researchers created a recombinant virus based on the Delta variant using vesicular stomatitis virus (VSV) technology, which proved effective for studying its properties and behavior in a safe laboratory environment.
- The new recombinant virus demonstrated strong replication in cell cultures and avirulence in mice, generating protective antibodies and showing potential as a valuable tool for researching the Delta variant, including vaccine development and understanding viral interactions.
Article Abstract
The frequent emergence of SARS-CoV-2 variants thwarts the prophylactic and therapeutic countermeasures confronting COVID-19. Among them, the Delta variant attracts widespread attention due to its high pathogenicity and fatality rate compared with other variants. However, with the emergence of new variants, studies on Delta variants have been gradually weakened and ignored. In this study, a replication-competent recombinant virus carrying the S protein of the SARS-CoV-2 Delta variant was established based on the vesicular stomatitis virus (VSV), which presented a safe alternative model for studying the Delta variant. The recombinant virus showed a replication advantage in Vero E6 cells, and the viral titers reach 10 TCID/mL at 36 h post-inoculation. In the VSV-vectored recombinant platform, the spike proteins of the Delta variant mediated higher fusion activity and syncytium formation than the wild-type strain. Notably, the recombinant virus was avirulent in BALB/c mice, Syrian hamsters, 3-day ICR suckling mice, and IFNAR/GR mice. It induced protective neutralizing antibodies in rodents, and protected the Syrian hamsters against the SARS-CoV-2 Delta variant infection. Meanwhile, the eGFP reporter of recombinant virus enabled the visual assay of neutralizing antibodies. Therefore, the recombinant virus could be a safe and convenient surrogate tool for authentic SARS-CoV-2. This efficient and reliable model has significant potential for research on viral-host interactions, epidemiological investigation of serum-neutralizing antibodies, and vaccine development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960918 | PMC |
| http://dx.doi.org/10.3390/microorganisms11020431 | DOI Listing |
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