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Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification. | LitMetric

Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification.

Micromachines (Basel)

Department of Electrical and Computer Engineering, University of Windsor, Windsor, ON N9B 3P4, Canada.

Published: February 2023

AI Article Synopsis

  • LAMP Technology Overview
  • : Loop-mediated isothermal amplification (LAMP) is gaining traction for quickly detecting pathogens in both clinical and environmental settings due to its sensitivity, speed, and suitability for portable devices.
  • Electrochemical Biosensors
  • : These sensors are emerging as a promising method for monitoring LAMP, offering advantages like low cost, high sensitivity, and the ability to integrate into small devices, compared to traditional methods like fluorescence or colorimetric techniques.
  • Detection Strategies
  • : The review discusses various voltammetric strategies for detecting products from the LAMP reaction, including direct binding of electroactive species to DNA and innovative approaches using aptamers for pyrophosphate, while also addressing challenges in making these

Article Abstract

Loop-mediated isothermal amplification (LAMP) is rapidly developing into an important tool for the point-of-use detection of pathogens for both clinical and environmental samples, largely due to its sensitivity, rapidity, and adaptability to portable devices. Many methods are used to monitor LAMP, but not all are amenable to point-of-use applications. Common methods such as fluorescence often require bulky equipment, whereas colorimetric and turbidimetric methods can lack sensitivity. Electrochemical biosensors are becoming increasingly important for these applications due to their potential for low cost, high sensitivity, and capacity for miniaturization into integrated devices. This review provides an overview of the use of voltammetric sensors for monitoring LAMP, with a specific focus on how electroactive species are used to interface between the biochemical products of the LAMP reaction and the voltammetric sensor. Various strategies for the voltammetric detection of DNA amplicons as well as pyrophosphate and protons released during LAMP are presented, ranging from direct DNA binding by electroactive species to the creative use of pyrophosphate-detecting aptamers and pH-sensitive oligonucleotide structures. Hurdles for adapting these devices to point-of-use applications are also discussed.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960872PMC
http://dx.doi.org/10.3390/mi14020472DOI Listing

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