Wheat germ oil (WGO) is the richest source of unexplored antioxidants and anti-inflammatory compounds. In this study, we identified the constituents of WGO by gas chromatography-mass spectrometry (GC-MS). The physicochemical and pharmacokinetic behaviors were evaluated for the top 12 constituents with the common target FABP4. Three fatty acids with significant anti-inflammatory activity were evaluated for their interaction with FABP4 by molecular docking. The molecular mechanisms involved in anti-inflammatory responses were analyzed by various analytical tools and multidimensional data analysis. WGO showed anti-inflammatory activities via FABP4 interacting physically with target genes (77.84%) and by co-expressing with 8.01% genes. Primary targets for inflammatory pathways were PPARα, PPARγ, LPL, LEP, and ADIPOQ, as depicted by gene network enrichment analysis. The key pathways implicated were the metabolism of lipids, PPAR signaling, cellular response to alcohol, oxygen and nitrogen pathway, inflammatory response pathway, and regulation of the inflammatory pathway. The common transcription factors implicated were HNF1, AP2α, CEBP, FOX, STATS, MYC, Zic, etc. In this study, we found that WGO possesses anti-inflammatory potential via FABP4 binding to PPARα, PPARγ, LPL, LEP, and ADIPOQ gene expression by regulatory transcription factors HNF, AP2α, and CEPB.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960255 | PMC |
| http://dx.doi.org/10.3390/life13020526 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Artificial Diet for Immatures of Vaurie, 1978 (Coleoptera: Curculionidae), Based on a Multidimensional Model.
Insects
November 2024
Department of Entomology and Acarology, "Luiz de Queiroz" College of Agriculture, University of São Paulo (USP), 11 Av. Pádua Dias, Piracicaba 13418-900, São Paulo, Brazil.
Pest attacks, especially by , are a factor affecting sugarcane production. As the pest's life habits make chemical control difficult, efforts are focused on finding integrated management alternatives. It is therefore essential to study the biology of , using artificial diets for rearing the insect in the laboratory.
View Article and Find Full Text PDFAdministration of Spermidine and Eugenol Demonstrates Anti-Tumorigenic Efficacy on Metastatic SW620 and Primary Caco-2 Colorectal Cancer Spheroids.
Int J Mol Sci
December 2024
Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, 40127 Bologna, Italy.
The anti-cancer potential of eugenol (EUG) is well recognized, whereas that of spermidine (SPD) is subject to dispute and requires further research. The anti-tumorigenic potential of wheat germ SPD (150 µM) and clove EUG (100 µM), alone, in combination as SPD+EUG (50 µM + 100 µM) and, as a supplement (SUPPL; 0.6 µM SPD + 50 µM EUG), was investigated on both metastatic SW620 and primary Caco-2 colorectal cancer (CRC) spheroids.
View Article and Find Full Text PDFEffects of cold-pressed wheat germ oil and Bacillus subtilis on growth performance, digestibility, immune status, intestinal microbial enumeration, and gene expression of broilers under heat stress.
Poult Sci
December 2024
Department of Biochemistry, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
This study evaluated the effect of wheat germ oil (WGO), Bacillus subtilis, and their combination on growth performance, immune response, nutrient digestibility, intestinal microbial, oxidative status, and gene expression in heat-stressed broilers. Four hundred one-day-old male Ross 308 broilers were distributed into five pens (20 birds/pen) in four experimental groups: a control (CON) without additives, WGO group fed diet with WGO at 200 mg.kg, BS group fed diet with B.
View Article and Find Full Text PDFPreparation, separation and identification of novel hypocholesterolemic peptides from wheat germ: An in vitro and in silico study.
Food Chem
December 2024
Engineering and Technology Center for Grain Processing of Shandong Province, Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Avenue, Tai'an 271018, China. Electronic address:
Article Synopsis
- The study aimed to extract and identify peptides from wheat germ protein that can lower cholesterol levels.
- Wheat germ protein was treated with various enzymes, and the most effective hydrolysate was isolated and tested for its ability to bind cholesterol and resist digestion.
- Three specific peptides were identified as effective in lowering cholesterol, showing significant solubility in cholesterol micelles, highlighting their potential use in functional foods.
Simultaneous Detection of Multiple Analytes at Ambient Temperature Using Eukaryotic Artificial Cells with Modular and Robust Synthetic Riboswitches.
ACS Synth Biol
December 2024
Proteo-Science Center, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime 790-8577, Japan.
Cell-free systems, which can express an easily detectable output (protein) with a DNA or mRNA template, are promising as foundations of biosensors devoid of cellular constraints. Moreover, by encasing them in membranes such as natural cells to create artificial cells, these systems can avoid the adverse effects of environmental inhibitory molecules. However, the bacterial systems generally used for this purpose do not function well at ambient temperatures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!