Proteome Analysis of Male Accessory Gland Secretions in the Diamondback Moth, (Lepidoptera: Plutellidae).

Insects

State Key Laboratory for Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Xianyang 712100, China.

Published: January 2023

In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs are transferred along with sperms inside female bodies and have a significant impact on the post-mating physiology changes of the females. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and vary from species to species. The diamondback moth, (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. Mating has a profound impact on the females' behavior and physiology in this species. It is still unclear what the ACPs are in this species. In this study, two different proteomic methods were used to identify ACPs in . The proteins of MAGs were compared immediately before and after mating by using a tandem mass tags (TMT) quantitative proteomic analysis. The proteomes of copulatory bursas (CB) in mated females shortly after mating were also analyzed by the shotgun LC-MS/MS technique. In total, we identified 123 putative secreted ACPs. Comparing with other four insect ACPs, trypsins were the only ACPs detected in all insect species. We also identified some new insect ACPs, including proteins with chitin binding Peritrophin-A domain, PMP-22/ EMP/ MP20/ Claudin tight junction domain-containing protein, netrin-1, type II inositol 1,4,5-trisphosphate 5-phosphatase, two spaetzles, allatostatin-CC, and cuticular protein. This is the first time that ACPs have been identified and analyzed in . Our results have provided an important list of putative secreted ACPs, and have set the stage for further exploration of the functions of these putative proteins in reproduction.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9960318PMC
http://dx.doi.org/10.3390/insects14020132DOI Listing

Publication Analysis

Top Keywords

male accessory
12
acps
11
accessory gland
8
diamondback moth
8
moth lepidoptera
8
lepidoptera plutellidae
8
putative secreted
8
secreted acps
8
insect acps
8
proteins
5

Similar Publications

Duodromic atrioventricular reentry tachycardia: a case report of a rare adenosine insensitive supraventricular tachycardia.

Eur Heart J Case Rep

January 2025

Arrhythmology, Pacing and Electrophysiology Unit, Cardiology Service, Santa Marta Hospital, Central Lisbon Hospital University Centre, R. de Santa Marta 50, Lisboa 1169-024, Portugal.

Background: Accessory pathways (AP) are associated with an increased risk of atrioventricular reentry tachycardia (AVRT), presenting as a wide QRS tachycardia if the mechanism is antidromic. Rarely, AVRT may not respond to adenosine, suggesting a duodromic mechanism if the patient has multiple APs. Herein, we present a case of a male patient with multiple APs, wide QRS complex tachycardia, and resistance to adenosine.

View Article and Find Full Text PDF

This study investigates the protective effects of resveratrol (RSV) against heat stress (HS)-induced testicular injury in rats. Climate change has exacerbated heat stress, particularly affecting male fertility by impairing testicular function and sexual behavior. A total of 32 rats were allocated into four experimental groups: control, RSV control, HS control, and RSV + HS.

View Article and Find Full Text PDF

Duplication of the Plantaris Tendon and Its Clinical Significance: A Case Report.

Cureus

December 2024

Orthopedics and Traumatology, Unidade Local de Saúde do Nordeste, Macedo de Cavaleiros, PRT.

The plantaris tendon may be absent in some individuals, indicating its unclear function. Anatomically, the plantaris tendon originates from the lateral femoral condyle and has a variable course and insertion point at the calcaneal tuberosity. The plantaris tendon may influence conditions such as Achilles tendinopathy, particularly in its midportion, whether by its close relation to the calcaneal tendon or adhesions between both tendons.

View Article and Find Full Text PDF

The role played by anionic channels in diabetic kidney disease (DKD) is not known. Chloride channel accessory 1 (CLCA1) facilitates the activity of TMEM16A (Anoctamin-1), a Ca2+-dependent Cl- channel. We examined if CLCA1/TMEM16A had a role in DKD.

View Article and Find Full Text PDF

Male seminal fluid allocation according to socio-sexual context in the South American fruit fly.

J Comp Physiol A Neuroethol Sens Neural Behav Physiol

January 2025

Laboratorio de Investigaciones Ecoetológicas de Moscas de la Fruta y sus Enemigos Naturales (LIEMEN), División Control Biológico de Plagas, PROIMI-Biotecnología, CONICET, Avenida Belgrano y Pasaje Caseros s/n, San Miguel de Tucumán, Tucumán, 4000, Argentina.

During copulation male insects transfer sperm and seminal fluids, including accessory gland proteins (Acps) to females, produced in the accessory glands (AGs). These Acps influence female behavior and physiology, inhibiting sexual receptivity, promoting ovulation and/or oviposition. The theory of ejaculate allocation postulates that production is costly; therefore, males strategically allocate ejaculates based on perception of sperm competition and quality and availability of females.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!