AI Article Synopsis

  • - Quality Protein Maize (QPM) has increased essential amino acids, particularly lysine and tryptophan, due to gene regulation affecting protein synthesis.
  • - The study identifies a specific SSR marker (phi112) that interacts with transcriptions factors and is linked to the quality of QPM, indicating a complex network of genetic influences.
  • - A multiplex PCR assay developed in the study can differentiate QPM from regular maize, supporting quality control in the production process.

Article Abstract

Quality Protein Maize (QPM) contains higher amounts of essential amino acids lysine and tryptophan. The QPM phenotype is based on regulating zein protein synthesis by transcription factor. Many gene modifiers act to optimize the amino acid content and agronomic performance. An SSR marker, phi112, is present upstream of the DNA gene. Its analysis has shown the presence of transcription factor activity. The functional associations of opaque2 have been determined. The putative transcription factor binding at phi112 marked DNA was identified through computational analysis. The present study is a step towards understanding the intricate network of molecular interactions that fine-tune the QPM genotype to influence maize protein quality. In addition, a multiplex PCR assay for differentiation of QPM from normal maize is shown, which can be used for Quality Control at various stages of the QPM value chain.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957476PMC
http://dx.doi.org/10.3390/genes14020531DOI Listing

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