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Genome Survey Sequencing of the Mole Cricket . | LitMetric

Genome Survey Sequencing of the Mole Cricket .

Genes (Basel)

College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.

Published: January 2023

AI Article Synopsis

  • The mole cricket is an important insect found globally, significant for its evolutionary, medicinal, and agricultural roles while living underground.
  • A study analyzed its genome size using flow cytometry and k-mer methods, estimating a haploid genome size of around 3.14 to 3.77 Gb, consistent with other insects in its suborder.
  • The research identified a high percentage of repetitive elements in the genome, with Class I-LINE retrotransposons being the most common; this information could aid in further taxonomic and biological studies of the species.

Article Abstract

The mole cricket is an evolutionarily, medicinal, and agriculturally significant insect that inhabits underground environments and is distributed globally. This study measured genome size by flow cytometry and k-mer based on low-coverage sequencing, and nuclear repetitive elements were also identified. The haploid genome size estimate is 3.14 Gb by flow cytometry, 3.17 Gb, and 3.77 Gb-based two k-mer methods, respectively, which is well within the range previously reported for other species of the suborder Ensifera. 56% of repetitive elements were found in , similar to 56.83% in . However, the great size of repetitive sequences could not be annotated to specific repeat element families. For the repetitive elements that were annotated, Class I-LINE retrotransposon elements were the most common families and more abundant than satellite and Class I-LTR. These results based on the newly developed genome survey could be used in the taxonomic study and whole genome sequencing to improve the understanding of the biology of .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957284PMC
http://dx.doi.org/10.3390/genes14020255DOI Listing

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