AI Article Synopsis
- The common dragonet is one of three species found in the North Sea, exhibiting significant sexual dimorphism, especially in males, which have distinctive coloration and size, while females are less distinguishable.
- A high-quality chromosome-level genome assembly was achieved using advanced sequencing techniques, resulting in an assembly length of 568.7 Mbp with a high level of completeness and over 19,000 annotated transcripts.
- This genome assembly will serve as an important reference for future studies in population genetics and evolutionary biology of the species and its relatives.
Article Abstract
Background: The common dragonet, , is one of three species inhabiting the North Sea. All three species show strong sexual dimorphism. The males show strong morphological differentiation, e.g., species-specific colouration and size relations, while the females of different species have few distinguishing characters. belongs to the 'benthic associated clade' of the order Syngnathiformes. The 'benthic associated clade' so far is not represented by genome data and serves as an important outgroup to understand the morphological transformation in 'long-snouted' syngnatiformes such as seahorses and pipefishes.
Findings: Here, we present the chromosome-level genome assembly of . We applied Oxford Nanopore Technologies' long-read sequencing, short-read DNBseq, and proximity-ligation-based scaffolding to generate a high-quality genome assembly. The resulting assembly has a contig N50 of 2.2 Mbp and a scaffold N50 of 26.7 Mbp. The total assembly length is 568.7 Mbp, of which over 538 Mbp were scaffolded into 19 chromosome-length scaffolds. The identification of 94.5% complete BUSCO genes indicates high assembly completeness. Additionally, we sequenced and assembled a multi-tissue transcriptome with a total length of 255.5 Mbp that was used to aid the annotation of the genome assembly. The annotation resulted in 19,849 annotated transcripts and identified a repeat content of 27.7%.
Conclusions: The chromosome-level assembly of provides a high-quality reference genome for future population genomic, phylogenomic, and phylogeographic analyses.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631949 | PMC |
| http://dx.doi.org/10.46471/gigabyte.6 | DOI Listing |
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