Transcriptomic Profiling of JEG-3 cells using human leiomyoma derived matrix.

Biomater Biosyst

Translational Immunology Research Program, and Department of Clinical and Medical Genetics, University of Helsinki, Helsinki 00290, Finland.

Published: August 2022

AI Article Synopsis

  • Oxygen levels change during pregnancy and can impact fetal development, with low oxygen linked to complications like growth restriction and preeclampsia.
  • Using JEG-3 cells as a trophoblast model, we examined how these cells respond to a uterine leiomyoma-derived matrix called Myogel, which closely mimics the uterine environment.
  • Our findings show that JEG-3 cells cultured on Myogel exhibit enhanced energy metabolism, increased ATP production, better cell adherence, and significant changes in gene expression related to oxygen usage and mitochondrial function.

Article Abstract

Oxygen tension varies during placental and fetal development. Although hypoxia drives early trophoblast invasion, low placental oxygen levels during pregnancy show association with pregnancy complications including fetal growth restriction and preeclampsia. JEG-3 cells are often used as a trophoblast model. We studied transcriptional changes of JEG-3 cells on a uterine leiomyoma derived matrix Myogel. This might be the closest condition to the real uterine environment that we can get for an in vitro model. We observed that culturing JEG-3 cells on the leiomyoma matrix leads to strong stimulation of ribosomal pathways, energy metabolism, and ATP production. Furthermore, Myogel improved JEG-3 cell adherence in comparison to tissue culture treated plastic. We also included PDMS microchip hypoxia creation, and observed changes in oxidative phosphorylation, oxygen related genes and several hypoxia genes. Our study highlights the effects of Myogel matrix on growing JEG-3 cells, especially on mitochondria, energy metabolism, and protein synthesis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934486PMC
http://dx.doi.org/10.1016/j.bbiosy.2022.100056DOI Listing

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