AI Article Synopsis
- - Researchers have created new optogenetic tools called BICYCLs, which are engineered to control protein-protein interactions (PPIs) using light of different colors, specifically red and green wavelengths.
- - BICYCLs allow for highly selective light control, with the ability to switch PPIs in a specific manner (green-ON/red-OFF or red-ON/green-OFF), which enhances the flexibility of these tools in experiments.
- - These tools can be used in mammalian cells to manipulate where proteins are located within the cell and can also work alongside existing blue-light optogenetic tools, expanding options for researchers.
Article Abstract
Optogenetic tools for controlling protein-protein interactions (PPIs) have been developed from a small number of photosensory modules that respond to a limited selection of wavelengths. Cyanobacteriochrome (CBCR) GAF domain variants respond to an unmatched array of colors; however, their natural molecular mechanisms of action cannot easily be exploited for optogenetic control of PPIs. Here we developed bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s by engineering synthetic light-dependent interactors for a red/green GAF domain. The systematic approach enables the future engineering of the broad chromatic palette of CBCRs for optogenetics use. BICYCLs are among the smallest optogenetic tools for controlling PPIs and enable either green-ON/red-OFF (BICYCL-Red) or red-ON/green-OFF (BICYCL-Green) control with up to 800-fold state selectivity. The access to green wavelengths creates new opportunities for multiplexing with existing tools. We demonstrate the utility of BICYCLs for controlling protein subcellular localization and transcriptional processes in mammalian cells and for multiplexing with existing blue-light tools.
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| http://dx.doi.org/10.1038/s41592-023-01764-8 | DOI Listing |
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Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s.
Nat Methods
March 2023
Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.
Article Synopsis
- - Researchers have created new optogenetic tools called BICYCLs, which are engineered to control protein-protein interactions (PPIs) using light of different colors, specifically red and green wavelengths.
- - BICYCLs allow for highly selective light control, with the ability to switch PPIs in a specific manner (green-ON/red-OFF or red-ON/green-OFF), which enhances the flexibility of these tools in experiments.
- - These tools can be used in mammalian cells to manipulate where proteins are located within the cell and can also work alongside existing blue-light optogenetic tools, expanding options for researchers.
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