The CRSIPR/Cas9 system has been applied to fission yeast, but there remain some rooms for improvement. Here we report that the weaker versions of the promoter, and promoters, for the potentially cytotoxic Cas9 achieved highly efficient mutagenesis and gene deletion at the locus. Employing a drug-selectable marker instead of conventional auxotrophic markers, our new vector system is compatible with a variety of experimental settings including prototrophic/auxotrophic strains and complete/minimal media.
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| http://dx.doi.org/10.17912/micropub.biology.000757 | DOI Listing |
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