Spirooxindole alkaloids feature a unique scaffold of an oxindole ring sharing an atom with a heterocyclic moiety. These compounds display an extensive range of biological activities such as anticancer, antibiotics, and anti-hypertension. Despite their structural and functional significance, the establishment and rationale of the spirooxindole scaffold biosynthesis are yet to be elucidated. Herein, we report the discovery and characterization of a cytochrome P450 enzyme from kratom () responsible for the formation of the spirooxindole alkaloids 3--corynoxeine (3, 7) and isocorynoxeine (3, 7) from the corynanthe-type (3)-secoyohimbane precursors. Expression of the newly discovered enzyme in yeast allows for the efficient and production of spirooxindoles. This discovery highlights the versatility of plant cytochrome P450 enzymes in building unusual alkaloid scaffolds and opens a gateway to access the prestigious spirooxindole pharmacophore and its derivatives.
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| http://dx.doi.org/10.3389/fpls.2023.1125158 | DOI Listing |
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