Neutrophils are the most abundant leukocytes in the bloodstream and are very important for the resolution of infection. One of the strategies used by neutrophils to eliminate a microorganism is the formation of extracellular traps. Different methods for neutrophil extracellular traps (NETs) visualization have been described along the years, usually requiring the use of a fluorescent, confocal or scanning electron microscope. This research aimed to visualize NETs using light microscopy as another way to study NETs prior to using the more expensive techniques, making NETs research more cost effective. We evaluated neutrophil purity, viability and function by analyzing the formation of NETs comparing DAPI with safranin. When evaluating NETs formation, neutrophils that were not stimulated did not form NETs and when neutrophils were exposed to PMA or NETs were formed and visualized with safranin under light microscopy and DAPI under fluorescence microscopy. Our method demonstrates another way to visualize NETs that can be added to the standard methods of visualization of NETs, increasing the opportunities to generate knowledge in the topic in any lab around the world.
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http://dx.doi.org/10.1016/j.bbrep.2023.101437 | DOI Listing |
BMC Genomics
January 2025
Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
Wool growth and fineness regulation is influenced by some factors such as genetics and environment. At the same time, lncRNA participates in numerous biological processes in animal production. In this research, we conducted a thorough analysis and characterization of the microstructure of wool, along with long non-coding RNAs (lncRNAs), their target genes, associated pathways, and Gene Ontology terms pertinent to the wool fineness development.
View Article and Find Full Text PDFEMBO J
January 2025
Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, Italy.
Endoplasmic reticulum (ER) plasticity and ER-phagy are intertwined processes essential for maintaining ER dynamics. We investigated the interplay between two isoforms of the ER-phagy receptor FAM134B in regulating ER remodeling in differentiating myoblasts. During myogenesis, the canonical FAM134B1 is degraded, while its isoform FAM134B2 is transcriptionally upregulated.
View Article and Find Full Text PDFArch Virol
January 2025
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing, Jiangsu, China.
In this study, a lytic phage, named PG216, was obtained from seawater collected in Qingdao, using Vibrio parahaemolyticus strain G299 as its host. Transmission electron microscopy revealed that phage PG216 has an icosahedral head with a diameter of 100 ± 6.7 nm and a contractible tail with a length of 126 ± 6.
View Article and Find Full Text PDFJ Imaging Inform Med
January 2025
State Key Laboratory of Traditional Chinese Medicine Syndrome/Health Construction Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
Nailfold microcirculation examination is crucial for the early differential diagnosis of diseases and indicating their severity. In particular, panoramic nailfold flow velocity measurements can provide direct quantitative indicators for the study of vascular diseases and technical support to assess vascular health. Previously, nailfold imaging equipment was limited by a small field of view.
View Article and Find Full Text PDFChin J Integr Med
January 2025
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Objective: To identify the underlying molecular mechanism of Modified Hu-Lu-Ba-Wan (MHW) in alleviating renal lesions in mice with diabetic kidney disease (DKD).
Methods: The db/db mice were divided into model group and MHW group according to a random number table, while db/m mice were settled as the control group (n=8 per group). The control and model groups were gavaged daily with distilled water [10 mL/(kg·d)], and the MHW group was treated with MHW [17.
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