AI Article Synopsis
- * The study investigates using surface enhanced Raman scattering microsensors (SERS-MS) to measure pH levels in patient-derived airway organoid cultures.
- * SERS-MS probes, made of polymer cores with gold nanoparticles and pH-sensitive molecules, can be incorporated into the organoids to provide real-time pH readings in both the organoid lumen and the surrounding extracellular matrix.
Article Abstract
Patient derived organoids have the potential to improve the physiological relevance of disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
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| http://dx.doi.org/10.1039/d2cc06582g | DOI Listing |
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