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http://dx.doi.org/10.1007/s00018-023-04701-2 | DOI Listing |
Sci Rep
December 2024
Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada.
J Pharm Sci
December 2024
Therapeutics Development & Supply, Janssen Research & Development, Beerse, Belgium.
Small interfering RNAs (siRNAs) have emerged as a highly promising class of therapeutics, capable of effectively treating a wide range of indications, including previously challenging targets. To correctly characterize the duplex content of siRNA therapeutics, a careful design of the analytical conditions is required. This is due to the weak interactions governing the duplex formation and thermal stability of these double-stranded oligonucleotides.
View Article and Find Full Text PDFElectrophoresis
December 2024
Chongqing Key Laboratory of Big Data for Bio Intelligence, Chongqing University of Posts and Telecommunications, Chongqing, China.
The identification of low-frequency variants remains challenging due to the inevitable high error rates of next-generation sequencing (NGS). Numerous promising strategies employ unique molecular identifiers (UMIs) for error suppression. However, their efficiency depends highly on redundant sequencing and quality control, leading to tremendous read waste and cost inefficiency.
View Article and Find Full Text PDFEnviron Sci Technol
December 2024
Department of Biology, University of Ottawa, Ottawa K1N 6N5, Canada.
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants that originate from the incomplete combustion of organic materials. We investigated the clastogenicity and mutagenicity of benzo[]fluoranthene (BbF), one of 16 priority PAHs, in MutaMouse males after a 28 day oral exposure. BbF causes robust dose-dependent increases in micronucleus frequency in peripheral blood, indicative of chromosome damage.
View Article and Find Full Text PDFBMC Urol
November 2024
Department of Urology, Weifang People's Hospital, Weifang, China.
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