Metabolic Engineering of for l-Malic Acid Production.

J Agric Food Chem

State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Published: March 2023

AI Article Synopsis

  • l-Malic acid, used in chemical and food industries, was produced efficiently through metabolic engineering of a filamentous fungus, creating an effective cell factory.
  • By overexpressing specific genes, including a C4-dicarboxylate transporter and pyruvate carboxylase, the researchers significantly increased l-malic acid production.
  • The engineered strain achieved a remarkable yield of 220.5 g/L in a 5 L fed-batch culture, marking the highest titer reported and preventing degradation of l-malic acid through gene deletion.

Article Abstract

l-Malic acid has various applications in the chemical and food industries. The filamentous fungus is known to be an efficient enzyme producer. Here, through metabolic engineering, was constructed for the first time as an excellent cell factory for l-malic acid production. The heterologous overexpression of genes encoding the C4-dicarboxylate transporter from and initiated l-malic acid production. The overexpression of pyruvate carboxylase from in the reductive tricarboxylic acid pathway further increased both the titer and yield of l-malic acid, resulting in the highest titer reported in a shake-flask culture. Furthermore, the deletion of malate thiokinase blocked l-malic acid degradation. Finally, the engineered strain produced 220.5 g/L of l-malic acid in a 5 L fed-batch culture (productivity of 1.15 g/L/h). A cell factory was created for the efficient production of l-malic acid.

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http://dx.doi.org/10.1021/acs.jafc.2c09078DOI Listing

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