Int J Food Microbiol
Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA. Electronic address:
Published: April 2023
Recent epidemiological evidence suggests that pork products may be vehicles for the transmission of Shiga toxin-producing Escherichia coli (STEC) to humans. The severe morbidity associated with STEC infections highlights the need for research to understand the growth behavior of these bacteria in pork products. Classical predictive models can estimate pathogen growth in sterile meat. However, competition models considering background microbiota reflect a more realistic scenario for raw meat products. The objective of this study was to estimate the growth kinetics of clinically significant STEC (O157, non-O157, and O91), Salmonella, and generic E. coli in raw ground pork using competition primary growth models at temperature abuse (10 and 25 °C) and sublethal temperature (40 °C). A competition model incorporating the No lag Buchanan model was validated using the acceptable prediction zone (APZ) method where >92 % (1498/1620) of the residual errors fell within the APZ (pAPZ > 0.70). The background microbiota (mesophilic aerobic plate counts, APC) inhibited the growth of STEC and Salmonella indicating a simple one-directional competitive interaction between pathogens and the mesophilic microbiota of ground pork. The maximum specific growth rate (μ) of all the bacterial groups was not significantly different (p > 0.05) based on fat content (5 vs 25 %) except for generic E. coli at 10 °C. E. coli O157 and non-O157 behaved similarly in terms of μ and maximum population density (MPD). Salmonella showed a similar (p > 0.05) μ to E. coli O157 and non-O157 at 10 and 40 °C but a significantly higher rate (p < 0.05) at 25 °C. STEC were more prone to be inhibited by APC than Salmonella at 10 and 25 °C. The μ of O91 was lower (p < 0.05) than other STEC and Salmonella at 10 and 25 °C but similar (p > 0.05) at 40 °C. Generic E. coli showed a two- to five-times higher (p < 0.05) μ (0.028 ± 0.011 log CFU/h) than other bacterial groups (0.006 ± 0.004 to 0.012 ± 0.003 log CFU/h) at 10 °C making it a potential indicator bacteria for process control. Industry and regulators can use competitive models to develop appropriate risk assessment and mitigation strategies to improve the microbiological safety of raw pork products.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2023.110134 | DOI Listing |
F1000Res
January 2025
Department of Human Pathology, University of Nairobi, Nairobi, Nairobi County, Kenya.
Background: Bacterial infections in the Intensive Care Units are a threat to the lives of critically ill patients. Their vulnerable immunity predisposes them to developing bacteria-associated sepsis, deteriorating their already fragile health. In the face of increasing antibiotics resistance, the problem of bacterial infection in ICU is worsening.
View Article and Find Full Text PDFLancet Reg Health Eur
March 2025
Department of Biostatistics, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands.
Background: It is unclear whether changes in antimicrobial resistance (AMR) in primary care influence AMR in hospital settings. Therefore, we investigated the dynamic association of AMR between primary care and hospitals.
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J Nanobiotechnology
January 2025
Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China.
The escalating hazards posed by bacterial infections underscore the imperative for pioneering advancements in next-generation antibacterial modalities and treatments. Present therapeutic methodologies are frequently impeded by the constraints of insufficient biofilm infiltration and the absence of precision in pathogen-specific targeting. In this current study, we have used chlorin e6 (Ce6), zeolitic imidazolate framework-8 (ZIF-8), polydopamine (PDA), and UBI peptide to formulate an innovative nanosystem meticulously engineered to confront bacterial infections and effectually dismantle biofilm architectures through the concerted mechanism of photodynamic therapy (PDT)/photothermal therapy (PTT) therapies, including in-depth research, especially for oral bacteria and oral biofilm.
View Article and Find Full Text PDFNat Microbiol
January 2025
Department of Molecular Microbiology, John Innes Centre, Norwich, UK.
Examples of long-range gene regulation in bacteria are rare and generally thought to involve DNA looping. Here, using a combination of biophysical approaches including X-ray crystallography and single-molecule analysis for the KorB-KorA system in Escherichia coli, we show that long-range gene silencing on the plasmid RK2, a source of multi-drug resistance across diverse Gram-negative bacteria, is achieved cooperatively by a DNA-sliding clamp, KorB, and a clamp-locking protein, KorA. We show that KorB is a CTPase clamp that can entrap and slide along DNA to reach distal target promoters up to 1.
View Article and Find Full Text PDFJ Biotechnol
January 2025
Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:
Promoters are crucial elements for controlling gene expression in cells, yet lactic acid bacteria (LAB) often lack a diverse set of available constitutive promoters with quantitative characterization. To enrich the LAB promoter library, this study focused on the known strong constitutive promoter P in LAB. Through error-prone PCR and dNTP analog-induced random mutagenesis, a library of 247 mutants of P was generated by using the red fluorescent protein (RFP) fluorescence intensity as a high-throughput screening indicator in Streptococcus thermophilus.
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