Solid-nanopores/nanopipettes have the exquisite ability to reveal the changes in molecular volume due to the advantages of adjustable size, good rigidity and low noise. Herein, a new platform for sensing application was established based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes. In this method, GQH was immobilized on gold-coated nanopipette, which could be used as a catalyst for the reaction of HO with 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to promote the conversion of ABTS to ABTS ions inside gold-coated nanopipette, and the change of transmembrane ion current could be monitored in real time. At the optimal conditions, there was a correlation between the ion current and the concentration of HO in a certain range, which could be used for the hydrogen peroxide sensing. The GQH immobilized nanopipette provides a useful platform to investigate enzymatic catalysis in confined environment, which can be used in electrocatalysis, sensing and fundamental electrochemistry.
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Article Synopsis
- * This nanoreactor integrates various components, including modified hyaluronic acid and guanosine, to generate reactive oxygen species (ROS) and increase hydrogen peroxide levels which damage cancer cells.
- * As a result, the nanoreactor effectively reduces cell survival by causing nuclear and mitochondrial damage while also depleting protective cellular components, leading to a strong anticancer effect in both lab experiments and living organisms.
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