Genomic organization and transcription of superoxide dismutase genes (, , and ) and response to diazinon toxicity in platyfish () by using SOD enzyme activity.

The aim of this study is to determine the effects of 50% of 96 h LC (5.25 ppm) diazinon on the expression of superoxide dismutase (SOD) enzyme genes (, , and ) and SOD enzyme activity at the end of 24, 48, 72, and 96 h in platyfish liver and gill tissues. To this end, we determined the tissue-specific distribution of , , and genes and performed analyses in platyfish (). It was determined that malondialdehyde (MDA) level and SOD enzyme activity were increased in the liver [(43.90 EU mg protein (control), 62.45 EU mg protein (24 h), 73.17 EU mg protein (48 h), 82.18 EU mg protein (72 h), 92.93 EU mg protein (96 h)] and gill [(16.44 EU mg protein (control), 33.47 EU mg protein (24 h), 50.38 EU mg protein (48 h), 64.62 EU mg protein (72 h), 74.04 EU mg protein (96 h)] tissues of platyfish exposed to diazinon, while the expression of the genes was down-regulated. The tissue-specific distribution of the genes varied, with the tissues and the genes expression were being predominant in the liver (628.32 in , 637.59 in , 888.5 in ). Thus, the liver was considered a suitable tissue for further gene expression studies. Based on the phylogenetic analyses, platyfish genes can be reported to be orthologs of / genes from other vertebrates. Identity/similarity analyses supported this determination. Conserved gene synteny proved that there are conserved genes in platyfish, zebrafish, and humans.