Customer requirements (CRs) are the essential driven forces of product development. Constrained by the rigid budget and time allocated to product development, much attentions and resources should be paid on critical customer requirements (CCRs). Product design occurs with an increasingly frenetic pace of change in today's competitive market, and the changes of external environment will lead to the changes of CRs. Thus, involving the sensitivity of CRs toward influence factors to identify CCRs is of great significance to grasp the directions of product evolution and enhance market competitiveness. To fill this gap, this study proposes a CCRs identification method integrated Kano model and structural equation model (SEM). First, the Kano model is adopted to determine the category of each CR. Second, based on CRs' categorization, an SEM model is established to measure the sensitivity of CRs toward the turbulence of influence factors. Then the importance of each CR is calculated, and by integrating the sensitivity and importance, a four-quadrant diagram is constructed to identify the CCRs. Finally, the identification of CCRs for smartphone is implemented as an example to demonstrate the feasibility and additional value of the proposed method.
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http://dx.doi.org/10.1007/s00163-023-00410-w | DOI Listing |
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January 2025
R Mitchell, Centre for Reproductive Health, Edinburgh, EH164TJ, United Kingdom of Great Britain and Northern Ireland.
Methods to quantify germ cell number in human immature testicular tissues are essential to evaluate the impact of chemotherapy exposures and for optimising cryopreservation protocols used in fertility preservation for prepubertal boys. Established quantification methods rely on the presence of round tubules within the tissue. However, round tubular cross sections are limited in human prepubertal testicular tissues, especially when using in vitro culture.
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January 2025
Department of Life Science, Faculty of Science, Gakushuin University, Mejiro, Tokyo, Japan.
Cytokinesis in animal and fungal cells requires the contraction of actomyosin-based contractile rings formed in the division cortex of the cell during late mitosis. However, the detailed mechanism remains incompletely understood. Here, we aim to develop a novel cell-free system by encapsulating cell extracts obtained from fission yeast cells within lipid vesicles, which subsequently leads to the formation of a contractile ring-like structure inside the vesicles.
View Article and Find Full Text PDFFASEB J
January 2025
August Krogh Section for Human and Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.
The kinases AMPK, and mTOR as part of either mTORC1 or mTORC2, are major orchestrators of cellular growth and metabolism. Phosphorylation of mTOR Ser1261 is reportedly stimulated by both insulin and AMPK activation and a regulator of both mTORC1 and mTORC2 activity. Intrigued by the possibilities that Ser1261 might be a convergence point between insulin and AMPK signaling in skeletal muscle, we investigated the regulation and function of this site using a combination of human exercise, transgenic mouse, and cell culture models.
View Article and Find Full Text PDFCurr Mol Med
January 2025
College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China.
Background: The global challenge of Multidrug-resistant Tuberculosis (MDR-TB) presents a substantial public health concern, requiring extended and complex treatment regimens. Understanding the factors impacting treatment results, particularly sputum culture conversion and Body Mass Index (BMI), is crucial. This retrospective cohort investigation conducted in Punjab, Pakistan, sought to explore the correlation between BMI and sputum culture conversion in individuals diagnosed with MDR-TB.
View Article and Find Full Text PDFWound Repair Regen
January 2025
Department of Orthopedic Surgery, University of Texas Health Science Center San Antonio, Texas, USA.
Our objective was to evaluate risk factors for re-infection in patients after treatment for diabetic foot osteomyelitis (OM). We used pooled patient level data from two RTCs that evaluated patients with diabetic foot infections. We evaluated 171 patients with OM.
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