Enterocytozoon hepatopenaei (EHP), an obligate intracellular parasite classified as microsporidia, is an emerging pathogen with a significant impact on the global shrimp aquaculture industry. The understanding of how microsporidia germinate has been a key factor in exploring its infection process. However, the germination process of EHP was rarely reported. To gain insight into the germination process, we conducted a high-throughput sequencing analysis of purified EHP spores that had undergone in vitro germination treatment. This analysis revealed 137 differentially expressed genes, with 84 up-regulated and 53 down-regulated genes. While the functions of some of the genes remain unknown, this study provides important data on the transcriptomic changes before and after EHP germination, which can aid in further studies on the EHP infection mechanism.
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Article Synopsis
- The microsporidian pathogen Enterocytozoon hepatopenaei (EHP) significantly impacts the shrimp industry, and understanding shrimp immune responses to it is limited.
- This study examined the prophenoloxidase (proPO)-activating system in Pacific white shrimp, revealing that it responds strongly to EHP infection by increasing melanin production and hemolymph activity, which helps combat the pathogen.
- Suppressing the proPO system led to heightened EHP levels in the shrimp and reduced effectiveness of other immune responses, highlighting its crucial role in fighting off EHP infections.
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