AI Article Synopsis
- The Golgi apparatus is vital for sorting proteins, especially in neurons, and this study investigates the localization of 21 proteins within it using advanced microscopy techniques.
- The researchers categorized the proteins based on their positioning relative to a key Golgi marker, TGN38, discovering three distinct groups: upstream, overlapping, and downstream of TGN38.
- Seven proteins were found to completely overlap with TGN38, while those downstream varied in distance from it, and upstream proteins were located between TGN38 and other Golgi markers, aligning with their known functions.
Article Abstract
The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we systematically compared protein localization of 21 endogenous proteins in the Golgi apparatus of mouse neurons using confocal microscopy and line scan analysis. We localized these proteins by measuring the distance relative to the canonical TGN marker TGN38. Based on this, proteins fell into three groups: upstream of, overlapping with or downstream of TGN38. Seven proteins showed complete overlap with TGN38, while proteins downstream of TGN38 were located at varying distances from TGN38. Proteins upstream of TGN38 were localized in between TGN38 and the cis-/medial Golgi markers Giantin and GM130. This localization was consistent with protein function. Our data provide an overview of the relative localization of endogenous proteins in the Golgi of primary mouse neurons.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938882 | PMC |
| http://dx.doi.org/10.1038/s41598-023-29998-8 | DOI Listing |
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