AI Article Synopsis
- A human induced pluripotent stem cell (hiPSC) line was created from blood cells of a female Parkinson's disease patient with specific mutations in the GBA gene.
- * The reprogramming used a non-integrative Sendai virus technique, resulting in the hiPSC line named GBA PD8 (PNUSCRi004-A).
- * This new cell line showed a normal chromosome structure and expressed markers indicating it can develop into all three germ layers: ectoderm, endoderm, and mesoderm.
Article Abstract
The hiPSC line was generated from peripheral blood mononuclear cells (PBMCs) collected from a female patient with young onset Parkinson's disease (PD), carrying on heterozygous c.1448 T > C (L483P), c1483 G > C (A495P) and c.1497 G > C (V499V) mutations in the GBA gene. The PBMCs was reprogrammed into an induced pluripotent stem cell (iPSC) line (GBA PD8 or PNUSCRi004-A hiPSCs) using non-integrative Sendai virus. The cell line, PNUSCRi004-A displayed a normal karyotype and expression of pluripotency markers capable of producing derivatives of three germ layers (Ectoderm, Endoderm and Mesoderm).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.scr.2023.103051 | DOI Listing |
Publication Analysis
Top Keywords
induced pluripotent
8
pluripotent stem
8
parkinson's disease
8
v499v mutations
8
characterization human
4
human induced
4
stem cells
4
cells pnuscri004-a
4
pnuscri004-a parkinson's
4
disease patient
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!