Glyphosate (GLY) is one of the most widely used agrochemicals in the world, and its exposure has become a public health concern. The freshwater planarian is an ideal test organism for detecting the toxicity of pollutants and has been an emerging animal model in toxicological studies. Nevertheless, the underlying toxicity mechanism of GLY to planarians has not been thoroughly explored. To elucidate the toxicity effects and molecular mechanism involved in GLY exposure of planarians, we studied the acute toxicity, histological change, and transcriptional response of Dugesia japonica subjected to GLY. Significant morphological malformations and histopathological changes were observed in planarians after GLY exposure for different times. Also, a number of differentially expressed genes (DEGs) were obtained at 1, 3 and 5 d after exposure; Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of these DEGs were performed, and a global and dynamic view was obtained in planarians upon GLY exposure at the transcriptomic level. Furthermore, real-time quantitative PCR (qRT-PCR) was conducted on nine DEGs associated with detoxification, apoptosis, stress response, DNA repair, etc. The expression patterns were well consistent with the RNA sequencing (RNA-seq) results at different time points, which confirmed the reliability and accuracy of the transcriptome data. Collectively, our results established that GLY could pose adverse effects on the morphology and histo-architecture of D. japonica, and the planarians are capable of responding to the disadvantageous stress by dysregulating the related genes and pathways concerning immune response, detoxification, energy metabolism, DNA damage repair, etc. To the best of our knowledge, this is the first report of transcriptomic analyses of freshwater planarians exposed to environmental pollutants, and it provided detailed sequencing data deriving from transcriptome profiling to deepen our understanding the molecular toxicity mechanism of GLY to planarians.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.aquatox.2023.106425 | DOI Listing |
Antioxidants (Basel)
November 2024
Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
is a low-molecular weight, non-aromatic reagent, widely used in industry, such as in the manufacture of paper, textiles, plastics, cosmetics, and dyes. ACR is formed during the cooking of starchy food and its toxicity results mainly by conferring oxidative stress by elevating reactive oxygen species (ROS). To identify potential antidotes for ACR toxicity, we evaluated the efficacy of several thiol-based molecules known for ROS-scavenging, disulfide-reducing properties, and inhibition of oxidative stress-induced activation of the mitogen-activated protein kinases (MAPKs): the extracellular-signal-regulated-kinases (ERK1/2), p38-mitogen-activated-protein-kinases (p38), and c-Jun-N-terminal-kinases (JNKs).
View Article and Find Full Text PDFSci Total Environ
January 2025
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquaculture Engineering and Technology Research Centre, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Glyphosate (Gly), the world's most widely used herbicide in agriculture, can poison the red swamp crayfish, Procambarus clarkii, via spray drift and surface runoff into surface waters. However, there is a paucity of research on the mechanisms that affect crayfish tolerance to Gly at typical environmental concentrations. To address this research gap, we investigated the effects of Gly stress (0, 6, 12, 24, and 72 h) at different concentrations (0, 1.
View Article and Find Full Text PDFAquac Nutr
December 2024
National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999 Huchenghuan Road, Shanghai 201306, China.
The use of glyphosate (Gly) has caused unnecessary economic losses to the aquaculture industry, but research on the effect of Gly on is very limited. The aim of this study is to reduce the negative effects of Gly, reduce yield loss, and improve economic benefits through nutritional feed control technology. The experiment involved 80 crabs randomly divided into four groups: control group, Gly group (48.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
Federal State Budgetary Educational Institution of Higher Education "St. Petersburg State Agrarian University", Pushkin, St. Petersburg 196601, Russia.
Studies have shown the presence of residual amounts of the herbicide glyphosate in poultry feed, which leads to its bioaccumulation in the body. Recently, it has been established that exposure to low levels of glyphosate over a long period may have serious negative effects on poultry health. Moreover, combined exposure to several toxicants can potentially lead to additive and/or synergistic effects.
View Article and Find Full Text PDFEnviron Pollut
December 2024
Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China. Electronic address:
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!