A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 +/- 1.8%) with a mean recovery of 23.3 +/- 6.4% (n = 6).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/oxfordjournals.humrep.a136591 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The mechanism of Se in regulating the proliferation and apoptosis of sheep Leydig cells through the miR-200a/NRF2 pathway.
Theriogenology
January 2025
College of Animal Science, Shanxi Agricultural University, Taigu, 030801, PR China; Laboratory of Animal Reproductive Biotechnology, Shanxi Agricultural University, Taigu, 030801, PR China. Electronic address:
This study aimed to investigate the mechanism by which Se in regulates the proliferation and apoptosis of sheep Leydig cells via the miR-200a/NRF pathway. The cells were isolated and purified from the testes of 8-month-old sheep via a Percoll density gradient. After the cells were treated with different concentrations of Se (0, 2.
View Article and Find Full Text PDFImpact of Post-Thaw Enrichment of Primary Human Hepatocytes on Steatosis, Inflammation, and Fibrosis in the TruVivo System.
Pharmaceuticals (Basel)
December 2024
Institute of Regenerative Medicine, LifeNet Health, VA Beach, VA 23453, USA.
: Liver diseases are a global health concern. Many in vitro liver models utilize cryopreserved primary human hepatocytes (PHHs), which commonly undergo post-thaw processing through colloidal silica gradients to remove debris and enrich for a viable PHH population. Post-thaw processing effects on healthy PHHs are partially understood, but the consequences of applying disease-origin PHHs to post-thaw density gradient separation have not been described.
View Article and Find Full Text PDFChemomechanical Self-Oscillatory Microgel Motility in Stratified Chemical Media.
Adv Mater
December 2024
Dynamic Colloidal Systems Laboratory, Department of Chemistry, Indian Institute of Technology, Roorkee, 247667, India.
The design of chemomechanical self-oscillators, which execute oscillations in the presence of constant stimuli lacking periodicity, is a step toward the development of autonomous and interactive soft robotic systems. This work presents a simple design of prolonged chemomechanical oscillatory movement in a microgel system capable of buoyant motility within stratified chemical media containing spatially localized sinking and floating stimuli. Three design elements are developed: a stimuli-responsive membranized calcium alginate microgel, a Percoll density gradient for providing stratified antagonistic chemical media, and transduction of microgel particle size actuation into buoyant motility via membrane-mediated displacement of the Percoll media.
View Article and Find Full Text PDFRole of water activity on sporulation traits and resistance to 915 MHz microwave in the emetic type of Bacillus cereus on rice.
Food Res Int
November 2024
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute of Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Institutes of Green BioScience & Technology, Seoul National University, Pyeongchang-gun, Gangwon-do 232-916, Republic of Korea. Electronic address:
The objective of this study is to investigate the influence of water activity on the sporulation of emetic strains of Bacillus cereus and the subsequent susceptibility of sporulated B. cereus to 915 MHz microwave treatment. Water activity levels were manipulated in the sporulation medium by adjusting glycerol concentrations to 0 %, 3 %, 7.
View Article and Find Full Text PDFSelenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells.
Reprod Biol
November 2024
College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR China; Laboratory of Animal Reproductive biotechnology, Shanxi Agricultural University, Taigu 030801, PR China. Electronic address:
The objective of this study was to investigate the effect of oxidative stress induced by excessive Se on autophagy of sheep Leydig cells and its underlying mechanism. Leydig cells isolated from the testis of 8-month-old sheep were purified using a discontinuous Percoll density gradient. Cells were divided into four treatment groups (0, 2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!