Many PCB-degrading aerobes have been identified which may serve as bioaugmentation strains for aerobic, in situ bioremediation or in combination with dredging operations. The present work describes a lab-scale PCB biodegradation assay which can be used to screen potential bioaugmentation strains or consortia for their ability to decrease PCB mass flux from contaminated sediment to air through biodegradation of freely dissolved PCBs that have desorbed from sediment particles. The assay uses two types of passive samplers to simultaneously measure PCB mass that is freely dissolved in aqueous solution and PCB mass that has volatilized to the headspace of the bioreactor. Using this approach, relative comparisons of PCB mass accumulated in passive samplers between bioaugmented treatments and controls allow for practical assessment of a microbial strain's ability to reduce both freely dissolved and vapor phase PCB concentrations. The method is designed to be conducted using aliquots of homogenized, well-characterized, PCB-contaminated sediment gathered from a field site. This work details the experimental design methodology, required materials, bioreactor set-up, passive sampling, PCB-extraction, sample cleanup, and quantification protocols such that the biodegradation assay can be conducted or replicated. A step-by-step protocol is also included and annotated with photos, tips, and tricks from experienced analysts.•Relative comparisons of PCB mass accumulated in passive samplers between experimental treatments and controls allow for practical assessment of bioaugmentation strain's ability to reduce both freely dissolved and vapor phase PCB concentrations•Passive sampler preparation, deployment, PCB-extraction, cleanup procedures, and quantification are detailed step-by-step and annotated by experienced analysts.
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http://dx.doi.org/10.1016/j.mex.2023.102039 | DOI Listing |
Sci Total Environ
January 2025
Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI, 48109, United States. Electronic address:
Polychlorinated naphthalenes (PCNs) and polychlorinated biphenyls (PCBs) are toxic contaminants that were produced and used in large quantities for their stability, inertness, and other desirable electrical, cooling, and lubricating properties. Due to their environmental persistence and improper disposal, these contaminants have become broadly distributed in the environment. This study examines the levels, composition, distribution, and potential sources of these compounds in surface soils and street dusts collected at 19 residential and industrial areas in Detroit, Michigan.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Department of Pain Management, Qilu Hospital of Shandong University, 107# West Wenhua Road, Jinan, Shandong 250012, China. Electronic address:
This investigation represents a pioneering effort to examine the therapeutic effects of PCB specifically in the context of CFA-induced mice, as well as to elucidate the underlying mechanisms that facilitate such effects. Our study utilized advanced methodologies, namely high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)-based metabolomics, alongside comprehensive multivariate data analysis, to identify a distinctive metabolic profile associated with acute inflammation. Through our analyses, we discovered that several potential metabolites were significantly implicated in a variety of critical metabolic pathways.
View Article and Find Full Text PDFLangmuir
January 2025
School of Engineering, Westlake University, Hangzhou, Zhejiang 310030, China.
Antifouling zwitterionic materials have extensive applications in the biomedical field. This study designed and successfully synthesized a novel poly(carboxybetaine) diacrylate (PCBDA) via cationic ring-opening polymerization of 2-methyl-2-oxazine, chain modification by the Michael reaction, and chain end transformation to acrylate. The cross-linker was obtained with a tunable molecular weight.
View Article and Find Full Text PDFJ Occup Environ Hyg
January 2025
Environmental Engineering Research Laboratory, Department of Chemical Engineering, University of Ilorin, Ilorin, Nigeria.
Cooking fuels are sources of polychlorinated biphenyls (PCBs), which are persistent in the environment and have detrimental effects on human health. Fifteen PCBs congeners from the smoke of eight (8) commonly used cooking fuels in Nigeria were investigated in this study. Glass fiber filters were used to collect air emissions during the combustion of cooking fuels in a controlled chamber.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
January 2025
Department of Chemistry, Washington State University, Pullman, Washington 99164, United States.
Phased structures for lossless ion manipulation offer significant improvements over the scanning second gate method for coupling with ion trap mass analyzers. With an experimental run time of under 1 min for select conditions and an average run time of less than 4 min, this approach significantly reduces experimental time while enhancing the temporal duty cycle. The outlined SLIM system connects to an ion trap mass analyzer via a PCB stacked ring ion guide, which replaces the commercial ion optics and capillary inlet.
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