Photolithographically defined thin film Au dots were used as micro fiducial markers for digital image correlation (DIC), to enable two-dimensional strain measurement of 200 m-thick LIGA (Lithographie, Galvanformung, Abformung) nickel alloys. Due to the sensitivity of electrodeposited films' microstructure and properties on the processing conditions, characterization of LIGA mechanical properties continues to be necessary for microsystems commercialization. DIC offers advantages over laser-based strain measurement techniques but creating suitable speckle patterns on specimens with dimensions under a millimeter is challenging. The material surface roughness itself is often used as the speckle pattern, or micro- or nanoparticles are applied to the surface. But for materials with highly polished surfaces, such as commercial LIGA alloys, the surface roughness is not always suitable, while application of particles still poses technical challenges in uniformity and reproducibility. We fabricated freestanding tensile specimens, with gauge sections 700 m wide × 3 mm long × 200 m thick, from electrodeposited Ni-10% Co using a commercial LIGA process, and conducted microtensile tests at strain rate 0.001 s. Designing and fabricating arrays of randomly oriented 1.5 m-thick Au dots on the specimens provided a suitable way to obtain full-field surface strains over the entire gauge lengths and was reproducible from one specimen to another. Microfabricated fiducial markers therefore can be a useful surface-preparation approach for investigating micromechanical behavior, particularly plasticity and fracture, of LIGA films using DIC.
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http://dx.doi.org/10.1088/2631-8695/abfb10 | DOI Listing |
Int J Radiat Oncol Biol Phys
January 2025
Department of Radiation Oncology, University of Florida College of Medicine, Jacksonville, FL, USA.
Objectives: Radiotherapy manages pancreatic cancer in various settings; however, the proximity of gastrointestinal (GI) luminal organs-at-risk (OAR) poses challenges to conventional radiotherapy. Proton beam therapy (PBT) may reduce toxicities compared to photon therapy. This consensus statement summarizes PBT's safe and optimal delivery for pancreatic tumors.
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January 2025
Bellvitge Biomedical Research Institute (IDIBELL), Hospitalet Barcelona, Barcelona, Spain.
Background And Purpose: The aim was to estimate the cost of the external beam radiotherapy (EBRT) in public health care centers in Catalonia (Spain), according to the ESTRO-HERO costing model for 2018.
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Nat Commun
January 2025
Department of Chemistry, New York University, New York, NY, USA.
Advancements in cryo-EM have stimulated a revolution in structural biology. Yet, for membrane proteins near the cryo-EM size threshold of approximately 40 kDa, including transporters and G-protein coupled receptors, the absence of distinguishable structural features makes image alignment and structure determination a significant challenge. Furthermore, resolving more than one protein conformation within a sample, a major advantage of cryo-EM, represents an even greater degree of difficulty.
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Newcastle Upon Tyne Hospitals NHS Foundation Trust, Northern Centre for Cancer Care, Newcastle Upon Tyne, United Kingdom; Newcastle University, Translational and Clinical Research Institute, Newcastle Upon Tyne, United Kingdom.
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View Article and Find Full Text PDFJ Struct Biol
December 2024
Biocomputing Unit, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin, 3, Campus Universidad Autonoma, 28049 Cantoblanco, Madrid, Spain. Electronic address:
Cryo-electron tomography is an imaging technique that allows the study of the three-dimensional structure of a wide range of biological samples, from entire cellular environments to purified specimens. This technique collects a series of images from different views of the specimen by tilting the sample stage in the microscope. Subsequently, this information is combined into a three-dimensional reconstruction.
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