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Evaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography.
Fish Physiol Biochem
February 2025
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and is directly associated with their metabolic state. The oxygen consumption rate (OCR) of spermatozoa reflects the respiratory capacity of sperm mitochondria under various physiological conditions and is an essential marker of sperm quality. We determined the OCR of common carp (Cyprinus carpio) sperm using two respirometry methods: the conventionally used polarographic method with a Clark-type electrode and fluorometric assay with an Oxo Dish optochemical oxygen sensor.
View Article and Find Full Text PDFObjective: Aim: To identify appropriate methods for determining the content of radioactive and non-radioactive pulmotoxic xenobiotics in the ambient air of NPP equipment to ensure its reliability, radiation and environmental safety, as well as to reduce the risks of occupational pathologies for workers and protect people's health.
Patients And Methods: Materials and Methods: Analytical methods of analysis of modern methods of determining the content of radioactive and non-radioactive pulmonary toxic xenobiotics in the ambient air of NPP equipment.
Conclusion: Conclusions: a) during operation of NPP equipment, pulmotoxic xenobiotics enter the surrounding air, which can then enter the human respiratory system in the form of radioactive or non-radioactive substances; b) signif i cant methods of determining the content of pulmotoxic xenobiotics in the air are: gas chroma-tography; gas chromatography-mass spectrometry; liquid scintillation; photometric, ionometric, polarographic, titrometric, turbidimetric, atomic absorption, radiometric and γ-spectrometric measurements; c) radioactive pulmonotoxic xenobiotics cause radiation pathologies in the respiratory organs as a result of internal radioactive irradiation of the body; d) the effects of non-radioactive pulmotoxic xenobiotics are accompanied by irritation and inf l ammatory processes in the respiratory organs, as well as toxic swelling of the lungs; е) there is a connection between the presence of pulmotoxic xenobiotics in the air and the course of human respiratory diseases as a result of breathing such air.
The impact of cannabinoid receptor 1 absence on mouse liver mitochondria homeostasis: insight into mitochondrial unfolded protein response.
Front Cell Dev Biol
October 2024
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania "L. Vanvitelli", Caserta, Italy.
Introduction: The contribution of Cannabinoid type 1 receptor (CB1) in mitochondrial energy transduction mechanisms and mitochondrial activities awaits deeper investigations. Our study aims to assess the impact of CB1 absence on the mitochondrial compartment in the liver, focusing on both functional aspects and remodeling processes.
Methods: We used CB1 and CB1 male mice.
Analysis of stallion spermatozoa metabolism using Agilent Seahorse XFp Technology.
Anim Reprod Sci
December 2024
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
Sperm metabolism consists of a sophisticated network of biochemical reactions and varies between species, resulting in different metabolic strategies for ATP production to maintain sperm functionality. ATP can be produced through glycolysis or in the mitochondria by oxidative phosphorylation (OXPHOS). Since OXPHOS is the predominant metabolic pathway in horses spermatozoa, various assessments of mitochondrial activity are used to evaluate fertility, utilizing techniques such as fluorescent probes analysed via microscopy or flow cytometry, and polarographic electrode assays to measure current flow in response to an applied voltage.
View Article and Find Full Text PDFAnalysis and characterization of sulfane sulfur.
Anal Biochem
April 2024
Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan. Electronic address:
In the late 1970s, sulfane sulfur was defined as sulfur atoms covalently bound only to sulfur atoms. However, this definition was not generally accepted, as it was slightly vague and difficult to comprehend. Thus, in the early 1990s, it was defined as "bound sulfur," which easily converts to hydrogen sulfide upon reduction with a thiol-reducing agent.
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