An impressive array of antigene approaches has been developed for recognition of double helical DNA over the past three decades; however, few have exploited the 'Watson-Crick' base-pairing rules for establishing sequence-specific recognition. One approach employs peptide nucleic acid as a molecular reagent and strand invasion as a binding mode. However, even with integration of the latest conformationally-preorganized backbone design, such an approach is generally confined to sub-physiological conditions due to the lack of binding energy. Here we report the use of a class of shape-selective, bifacial nucleic acid recognition elements, namely Janus bases, for targeting double helical DNA or RNA. Binding occurs in a highly sequence-specific manner under physiologically relevant conditions. The work may provide a foundation for the design of oligonucleotides for targeting the secondary and tertiary structures of nucleic acid biopolymers.
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http://dx.doi.org/10.1038/s42004-018-0080-5 | DOI Listing |
Arch Microbiol
January 2025
Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, NH17B, Zuarinagar, Goa, 403726, India.
The gene gp13 in bacteriophage Phi11 has been annotated as a Single-Stranded DNA binding protein (SSB protein, GenBank accession no. NC_004615.1).
View Article and Find Full Text PDFAnal Chem
January 2025
The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310022, China.
Swift and efficient enrichment and isolation of extracellular vesicles (EVs) are crucial for enhancing precise disease diagnostics and therapeutic strategies, as well as elucidating the complex biological roles of EVs. Conventional methods of isolating EVs are often marred by lengthy and laborious processes. In this study, we introduce an innovative approach to enrich and isolate EVs by leveraging the capabilities of DNA nanotechnology.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
State Key Laboratory of Green Pesticide, Central China Normal University, Wuhan 430079, PR China. Electronic address:
The construction of helical structures through self-assembly and the exploration of their formation mechanisms not only amplify chiroptical properties but also provide profound insights into the structures and functions of natural helices. In this study, we developed a chiral Au(I) system based on BINAP and alkynyl ligands. The modification of the length or number of alkyl chains at the terminal positions of the alkynyl ligands significantly impacted the self-assembly behavior of the complexes.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address:
This study investigated the effects of Chlamydomonas reinhardtii polysaccharides (CRPs) on retarding the retrogradation of japonica rice starch (JS) and glutinous rice starch (GS). Structure characterization revealed that CRPs, with an average molecular weight of 505 kDa, mainly consisted of glucose, mannose, and galactose and featured a triple-helix structure. CRPs could reduce the storage modulus increment of JS during the cooling process by interacting with amylose, thereby inhibiting gel network formation.
View Article and Find Full Text PDFJ Contemp Dent Pract
September 2024
Department of Prosthodontics and Crown & Bridge, Bharati Vidyapeeth Deemed to be University, Dental College and Hospital, Pune, Maharashtra, India, ORCID: https://orcid.org/0009-0008-7338-1699.
Aim: This study aimed to compare the mixing efficacy and cost-effectiveness of new T-mixer tips against the standard double helical tips for a light-body elastomeric impression and a temporary/interim restorative material using a scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy.
Methodology: Automixed samples ( = 16) were divided into four groups of four samples each: Samples that were mixed with Helical tip for elastomer, T-mixer tip for elastomer, Helical tip for interim restorative material, and T-mixer tip for interim restorative material. These samples were then evaluated for SEM analysis.
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