Geogenic fluoride contaminates the water of tens of millions of people. However, many are unaware of the fluoride content due in part to shortcomings of detection methods. Biosensor tests are a relatively new approach to water quality testing that address many of these shortcomings but have never been tested by non-experts in a "real-world" setting. We therefore sought to assess the accuracy and usability of a point-of-use fluoride biosensor using surveys and field tests in Nakuru County, Kenya. Biosensor tests accurately classified elevated fluoride (≥1.5 ppm) in 89.5% of the 57 samples tested. Usability was also high; all participants were able to use the test and correctly interpreted all but one sample. These data suggest that biosensor tests can provide accurate, meaningful water quality data that help non-experts make decisions about the water they consume. Further scaling of these technologies could provide new approaches to track global progress towards Sustainable Development Goal 6.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905762PMC
http://dx.doi.org/10.1038/s41545-023-00221-5DOI Listing

Publication Analysis

Top Keywords

biosensor tests
12
accuracy usability
8
usability point-of-use
8
point-of-use fluoride
8
water quality
8
fluoride
5
fluoride biosensors
4
biosensors rural
4
rural kenya
4
kenya geogenic
4

Similar Publications

Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva.

Sensors (Basel)

January 2025

Department of Clinical and Molecular Biochemistry, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp. Al., 70-111 Szczecin, Poland.

Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements.

View Article and Find Full Text PDF

A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in .

Microorganisms

December 2024

Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Carrer de les Sitges, s/n, 08193 Bellaterra, Catalonia, Spain.

The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids and fatty alcohols. However, measuring subcellular concentrations of these metabolites remains technically challenging. Here, we explored 3-hydroxypropionic acid (3-HP), readily quantifiable and sharing the same precursors-acetyl-CoA, NADPH, and ATP-with the above-mentioned products, as a reporter metabolite for peroxisomal engineering in the yeast .

View Article and Find Full Text PDF

Aptamer-antibody sandwich immunosensor for electrochemical detection of FT4.

Mikrochim Acta

January 2025

College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, 26 Yuxiang Road, Shijiazhuang, 050018, P. R. China.

An aptamer-antibody sandwich electrochemical immunosensor was studied. FeO/MWCNTs-COOH/Nafion was modified and fixed on a glassy carbon electrode to amplify electrical signals. The antibody was coupled with AuNPs to form conjugates.

View Article and Find Full Text PDF

Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing - Opportunities and challenges.

Bioelectrochemistry

January 2025

LAQV-REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Electronic address:

The ability of the living world to flourish in the face of constant exposure to dangerous chemicals depends on the management ability of a widespread group of enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters of all reactions determining the metabolism of endogenous compounds, of those carried in foods, of taken drugs, or even of synthetic chemicals discarded into the environment depend on their catalytic performance. The chromatographic and (photo)luminometric methods routinely used as predictive and analytical tools in laboratories have significant drawbacks ranging from limited shelf-life of reagents, use of synthetic substrates, laborious and tedious procedures for highly sensitive detection.

View Article and Find Full Text PDF

Is Breath Best? A Systematic Review on the Accuracy and Utility of Nanotechnology Based Breath Analysis of Ketones in Type 1 Diabetes.

Biosensors (Basel)

January 2025

NanoTech Laboratory, School of Engineering, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.

Timely ketone detection in patients with type 1 diabetes mellitus (T1DM) is critical for the effective management of diabetic ketoacidosis (DKA). This systematic review evaluates the current literature on breath-based analysis for ketone detection in T1DM, highlighting nanotechnology as a potential for a non-invasive alternative to blood-based ketone measurements. A comprehensive search across 5 databases identified 11 studies meeting inclusion criteria, showcasing various breath analysis techniques, such as semiconducting gas sensors, colorimetry, and nanoparticle-based chemo-resistive sensors.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!